Human T cell lines CEM.NKR, CEM-SS, and CEM-T4 were obtained from the NIH AIDS Research and Reference Reagent Program. CEM.NKR subclones N2-NP, N5-P, and N8-SP, and the CEM-SS-derived SS-R5 cells were described before (Zhou et al., 2012 (link); Zhou et al., 2009 (link)). All these human T cells were cultured in RPMI 1640 with 10% fetal bovine serum (HyClone). The human 293T cell line was purchased from ATCC and cultured in DMEM with 10% bovine calf serum (HyClone). Human peripheral blood mononuclear cells (PBMC) were purchased from Gulf Coast Regional Blood Center. CD14+ monocytes were purified from PBMC using CD14 antibody-coated magnetic beads (Miltenyi Biotec, Germany). MDDC was generated by stimulating monocytes with 100 ng/ml GM-CSF and 50 ng/ml IL-4 for 7 days, and MDM was generated by stimulating monocytes with 100 ng/ml GM-CSF for 7 days.
Cd14 antibody coated magnetic beads
Manufactured by Miltenyi Biotec
Sourced in Germany
CD14 antibody-coated magnetic beads are a laboratory tool designed for the isolation and purification of CD14-positive cells from biological samples. These beads are coated with specific antibodies that bind to the CD14 surface antigen, enabling the separation and enrichment of CD14-expressing cells using a magnetic field.
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Lab products found in correlation
2 protocols using cd14 antibody coated magnetic beads
1
Human T Cell Line Cultivation and Derivation
2
Isolation and Differentiation of Monocyte-Derived Dendritic Cells
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Human peripheral blood mononuclear cells (PBMCs) from healthy donor were purchased from Blood Center of Shanghai (Shanghai, China). CD14+ monocytes were isolated from PBMCs by using CD14 antibody-coated magnetic beads (Miltenyi Biotec, Germany) as previously described [54] (link). Monocytes were differentiated into MDDCs by stimulation with 50 ng/ml of GM-CFS and IL-4 for 6 days. HEK293T cell line was kindly gifted by Dr. Li Wu (The Ohio State University, Columbus, OH, USA) and HeLa-cell-derived Magi/CCR5 cell line[39] (link) was a gift from Dr. Paul Zhou (Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China).
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