The 1 mg mL−1 solutions of the single 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, ≥99%, Sigma, St. Louis, MI, USA), cyclosporine A (CsA, ≥99%, Alfa Aesar, Kandel, Germany), lauryl gallate (LG, ≥99%, Aldrich, St. Louis, MI, USA), binary DOPC-CsA 0.50 (at the 1:1 molar ratio), and ternary DOPC-CsA-LG (the LG molar fraction equal to 0.25, 0.50, 0.75) were obtained. The binary and ternary solutions were prepared by mixing proper amounts of the single ones dissolved in 4:1, v:v chloroform:methanol (chloroform 99.8%, Macron Fine Chemicals, methanol ≥99.9%, FlukaTM) solvents.
Cyclosporine a
Manufactured by Thermo Fisher Scientific
Sourced in United States
Cyclosporine A is a powerful immunosuppressant drug widely used in organ transplant procedures. It functions by inhibiting the activity of calcineurin, a key enzyme involved in the activation of T-cells, thereby suppressing the immune response and reducing the risk of graft rejection.
Automatically generated - may contain errors
Lab products found in correlation
Lauryl gallate, by Merck Group (3 mentions)
Chloroform, by Merck Group (1 mentions)
Antimycin a, by Thermo Fisher Scientific (1 mentions)
Necrostatin 1 nec 1, by Enzo Life Sciences (1 mentions)
Poly l lysine (pll), by Merck Group (1 mentions)
Q vd oph hydrate, by Apexbio (1 mentions)
Taqman snp genotyping assay, by Thermo Fisher Scientific (1 mentions)
Cas9 mrna, by TriLink (1 mentions)
6 protocols using cyclosporine a
1
Preparation of DOPC-CsA-LG Lipid Solutions
2
Lipid-Drug Formulations for Controlled Release
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The proper amount of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, ≥99%, Sigma, St. Louis, MO, USA), cyclosporine A (CsA, ≥99%, Alfa Aesar, Kandel, Germany), and lauryl gallate (LG, ≥99%, Aldrich, St. Louis, MO, USA) was dissolved in the chloroform:methanol (4:1, v:v) mixture to obtain the final concentration equal to 1 mg mL−1. Chloroform (99.8%) and methanol (≥99.9%) were purchased from Macron Fine Chemicals and from FlukaTM, respectively. Then, by mixing the basic solutions, binary (DOPC-CsA with the 1:1 molar ratio) and ternary (DOPC-CsA-LG with the LG molar fraction ( ) equal to 0.25, 0.50, 0.75) ones were obtained.
3
Lipid Vesicle Formulations with Cyclosporine A and Lauryl Gallate
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1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC, ≥99%, Sigma), cyclosporine
A (CsA, ≥99%, Alfa Aesar), and lauryl gallate (LG, ≥99%,
Aldrich) were used as received. Stock solutions (1 mg mL–1) of these compounds were prepared in chloroform/methanol (4:1, v/v)
solutions (chloroform, Macron Fine Chemicals, 99.8%; methanol, Fluka,
≥99.9%). Then, by mixing appropriate volumes of the stock solutions,
the binary (DOPC–CsA 0.50) and ternary (DOPC–CsA–LG
0.25, 0.50, 0.75) mixtures of different molar ratios of components
were prepared (keeping a DOPC/CsA ratio of 1:1, where the numbers
denote the molar fraction of the LG component). All solutions were
stored in dark glass bottles to protect them from light-induced damage.
A (CsA, ≥99%, Alfa Aesar), and lauryl gallate (LG, ≥99%,
Aldrich) were used as received. Stock solutions (1 mg mL–1) of these compounds were prepared in chloroform/methanol (4:1, v/v)
solutions (chloroform, Macron Fine Chemicals, 99.8%; methanol, Fluka,
≥99.9%). Then, by mixing appropriate volumes of the stock solutions,
the binary (DOPC–CsA 0.50) and ternary (DOPC–CsA–LG
0.25, 0.50, 0.75) mixtures of different molar ratios of components
were prepared (keeping a DOPC/CsA ratio of 1:1, where the numbers
denote the molar fraction of the LG component). All solutions were
stored in dark glass bottles to protect them from light-induced damage.
4
Synthesis and Characterization of Y100 and Y100B
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Y100 and Y100B were synthesized by Chembridge Corp (San Diego, CA). The synthesis of Y100 and Y100B was first described by Gornostaev and Lavrikova [59 ]. Necrostatin-1 (Nec-1) and buthionine sulfoximine (BSO) were purchased from Enzo Life Sciences (Farmingdale, NY), Q-VD-OPh hydrate (QVD) was purchased from ApexBio (Houston, TX), GSK’872 (RIPK3 inhibitor) and oligomycin (A/B/C) were purchased from Calbiochem/EMD Millipore (Billerica, MA), carbonyl cyanide m-chlorophenylhydrazone (CCCP), hydroxyurea (HU), and poly-L-lysine were purchased from Sigma-Aldrich (St. Louis, MO), antimycin A and cyclosporine A were purchased from Alfa Aesar (Haverhill, MA), and hydroxychloroquine sulfate (HCQ) was purchased from Spectrum Chemicals (New Brunswick, NJ). Stock solutions of all molecules were prepared in 100% DMSO with the exception of HCQ and HU, which were prepared in phosphate buffered saline without calcium and magnesium (Corning #21-040-CV).
5
Humanized Myh6 Mouse Model of HCM
6
Lipid-Drug Interactions: Mixing Protocols
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1,2-Dioleoyl-sn-glycero-3-phosphocholine
(DOPC, ≥99%, Sigma), cyclosporine A (CsA, ≥99%, Alfa
Aesar), and lauryl gallate (LG, ≥99%, Aldrich) were used as
received. The appropriate amounts of the above compounds were dissolved
in a chloroform/methanol (4:1, v/v) mixture to obtain a final concentration
of 1 mg/mL. Chloroform was purchased from Macron Fine Chemicals (99.8%)
and methanol from Fluka (≥99.9%). Then, the binary (DOPC–LG,
CsA–LG, DOPC–CsA) and ternary (DOPC–LG–CsA,
CsA–LG–DOPC, DOPC–CsA–LG) systems were
prepared by mixing proper volumes of basic solutions so as to receive
the molar fractions of the second or third component, respectively,
equal to 0.25, 0.50, and 0.75. In addition, for the ternary mixtures,
the constant molar ratio of two components 1:1 was maintained.
(DOPC, ≥99%, Sigma), cyclosporine A (CsA, ≥99%, Alfa
Aesar), and lauryl gallate (LG, ≥99%, Aldrich) were used as
received. The appropriate amounts of the above compounds were dissolved
in a chloroform/methanol (4:1, v/v) mixture to obtain a final concentration
of 1 mg/mL. Chloroform was purchased from Macron Fine Chemicals (99.8%)
and methanol from Fluka (≥99.9%). Then, the binary (DOPC–LG,
CsA–LG, DOPC–CsA) and ternary (DOPC–LG–CsA,
CsA–LG–DOPC, DOPC–CsA–LG) systems were
prepared by mixing proper volumes of basic solutions so as to receive
the molar fractions of the second or third component, respectively,
equal to 0.25, 0.50, and 0.75. In addition, for the ternary mixtures,
the constant molar ratio of two components 1:1 was maintained.
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