All images were obtained using a Nikon Eclipse Ti-E inverted epifluorescent microscope equipped with a motorized stage. Images were acquired in NIS-Elements software (Nikon) with a 10x/0.3NA PlanFluor objective. The ND acquisition menu within Elements was used to take images from adjacent fields of view and digitally stitch them (with 15% overlap) to form a single image of the entire muscle cross-section (approximately 20–30 mm2) used for analysis. For our analyses, all images were acquired as 16-bit multi-channel images, which open in Fiji as 16-bit hyperstacks. Myosoft will work with 8 or 16-bit images, but images must be formatted as hyperstacks prior to analysis. We provide an ImageJ macro for converting single channel images into a Myosoft-compatible hyperstack in S2 File .
Eclipse ti e inverted epifluorescent microscope
Manufactured by Nikon
The Eclipse Ti-E inverted epifluorescent microscope is a high-performance laboratory equipment designed for advanced imaging applications. It features a motorized inverted stand and supports a range of fluorescence imaging techniques. The microscope is capable of capturing detailed images and data for research and analysis purposes.
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Lab products found in correlation
2 protocols using eclipse ti e inverted epifluorescent microscope
1
Large-Scale Muscle Imaging and Analysis
2
Multimodal Flow Cytometry and Microscopy
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A Becton Dickinson Accuri™ C6 outfitted with a 488 nm laser and a 552 nm laser were used for all flow cytometry analysis. Further data processing was performed using FlowJoTM software. A Nikon Eclipse Ti-E inverted epifluorescent microscope, with an Andor® Zyla scientific CMOS (sCMOS) camera mounted for image acquisition, was used for fluorescence imaging and Nikon’s NIS-Elements software was used for capturing and analyzing images.
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