The largest database of trusted experimental protocols

Magmax for microarrays total rna isolation kit

Manufactured by Thermo Fisher Scientific

The MagMAX for Microarrays Total RNA Isolation Kit is a product designed to extract total RNA from a variety of sample types. It utilizes magnetic bead-based technology to capture and purify the RNA, which can then be used for downstream applications such as microarray analysis.

Automatically generated - may contain errors

4 protocols using magmax for microarrays total rna isolation kit

1

Zika Virus Detection in Florida

Check if the same lab product or an alternative is used in the 5 most similar protocols
Clinical samples from locally-acquired ZIKV infections were collected from June 22 to October 11, 2016. The Florida DOH identified persons with compatible illness and clinical samples were shipped to the Bureau of Public Health Laboratories for confirmation by qRT-PCR and antibody tests following interim guidelines3 (link),38 (link)–40 (link). Clinical specimens (whole blood, serum, saliva, or urine) submitted for analysis were refrigerated or frozen at ≤ −70°C until RNA was extracted. RNA was extracted using the RNAeasy kit (QIAGEN), MagMAX for Microarrays Total RNA Isolation Kit (Ambion), or MagNA Pure LC 2.0 or 96 Systems (Roche Diagnostics). Purified RNA was eluted into 50–100 μL using the supplied elution buffers, immediately frozen at ≤ −70°C, and transported on dry ice. The Florida DOH also provided investigation data for these samples, including symptom onset dates and, when available, assignments to the zone where infection likely occurred (Supplementary Table 1).
+ Open protocol
+ Expand
2

Zika Virus Surveillance in Miami-Dade Mosquitoes

Check if the same lab product or an alternative is used in the 5 most similar protocols
24,351 Ae. aegypti and Ae. albopictus mosquitoes (sorted into 2,596 pools) were collected throughout Miami-Dade County during June to November, 2016 using BG-Sentinel mosquito traps (Biogents AG). Up to 50 mosquitoes of the same species and sex were pooled per trap. The pooled mosquitoes were stored in RNAlater (Invitrogen), RNA was extracted using either the RNAeasy kit (QIAGEN) or MagMAX for Microarrays Total RNA Isolation Kit (Ambion), and ZIKV RNA was detected by qRT-PCR targeting the envelope protein coding region40 (link) or the Trioplex qRT-PCR kit41 (link). ZIKV infection rates were calculated per 1,000 female Ae. aegypti mosquitoes using the bias-corrected maximum likelihood estimate (MLE)42 . Days of insecticide usage by the Miami-Dade Mosquito Control were inferred from the zone-specific ZIKV activities timelines published by the Florida DOH35 .
+ Open protocol
+ Expand
3

Zika Virus Surveillance in Miami-Dade Mosquitoes

Check if the same lab product or an alternative is used in the 5 most similar protocols
24,351 Ae. aegypti and Ae. albopictus mosquitoes (sorted into 2,596 pools) were collected throughout Miami-Dade County during June to November, 2016 using BG-Sentinel mosquito traps (Biogents AG). Up to 50 mosquitoes of the same species and sex were pooled per trap. The pooled mosquitoes were stored in RNAlater (Invitrogen), RNA was extracted using either the RNAeasy kit (QIAGEN) or MagMAX for Microarrays Total RNA Isolation Kit (Ambion), and ZIKV RNA was detected by qRT-PCR targeting the envelope protein coding region40 (link) or the Trioplex qRT-PCR kit41 (link). ZIKV infection rates were calculated per 1,000 female Ae. aegypti mosquitoes using the bias-corrected maximum likelihood estimate (MLE)42 . Days of insecticide usage by the Miami-Dade Mosquito Control were inferred from the zone-specific ZIKV activities timelines published by the Florida DOH35 .
+ Open protocol
+ Expand
4

Zika Virus Detection in Florida

Check if the same lab product or an alternative is used in the 5 most similar protocols
Clinical samples from locally-acquired ZIKV infections were collected from June 22 to October 11, 2016. The Florida DOH identified persons with compatible illness and clinical samples were shipped to the Bureau of Public Health Laboratories for confirmation by qRT-PCR and antibody tests following interim guidelines3 (link),38 (link)–40 (link). Clinical specimens (whole blood, serum, saliva, or urine) submitted for analysis were refrigerated or frozen at ≤ −70°C until RNA was extracted. RNA was extracted using the RNAeasy kit (QIAGEN), MagMAX for Microarrays Total RNA Isolation Kit (Ambion), or MagNA Pure LC 2.0 or 96 Systems (Roche Diagnostics). Purified RNA was eluted into 50–100 μL using the supplied elution buffers, immediately frozen at ≤ −70°C, and transported on dry ice. The Florida DOH also provided investigation data for these samples, including symptom onset dates and, when available, assignments to the zone where infection likely occurred (Supplementary Table 1).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!