Donkey anti rat alexa 488

For immunohistochemistry and confocal microscopy, mice were perfused with 1 × PBS (pH 7.4), under ketamine/xylazine/acepromazine maleate anesthesia (75/10/2 mg/kg body weight, bolus injection) and decapitated. Brains were removed from the skull afterwards, fixed in 4 % paraformaldehyde in 1 × PBS (pH 7.4). Brain tissue were cut horizontally at 5 μm and stained with CRANAD-2, anti-Aβ₁₋₁₆ antibody 6E10 (Signet Lab, SIG-39320, 1:5000), fibrillar conformation anti-amyloid antibody OC (Merck, AB2286, 1:200), Donkey-anti-Rat Alexa 488 (Jackson, AB-2340686, 1:400), Goat-anti-Rabbit Alexa488 (Invitrogen, A11034, 1:200) and counter-stained using 4′,6-diamidino-2-phenylindole (DAPI) for nuclei (Sigma, D9542−10MG, 1:1000). Confocal images of the cortex of non-transgenic littermates and arcAβ mice were obtained using a Leica SP8 confocal microscope (Leica Microsystems GmbH, Germany) at ScopeM ETH Zurich Hönggerberg core facility. Sequential images were obtained by using 458 nm, 640 nm lines of the laser respectively. Identical settings were used and images were obtained for the non-transgenic littermates and arcAβ mice at ×20 and ×60 magnification, resolution with Z stack and maximum intensity projection.