Sq detector

Manufactured by Waters Corporation

Sourced in United States

The SQ Detector is a high-performance mass spectrometer designed for a wide range of analytical applications. It provides accurate and reliable mass detection capabilities to support various laboratory workflows.

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Lab products found in correlation

Acquity uplc system, by Waters Corporation (2 mentions) Xbridge c18 chromatographic column, by Waters Corporation (1 mentions) Systane ultra, by Alcon (1 mentions) Luna c18 2 100 column, by Phenomenex (1 mentions) Evolution 260 bio spectrophotometer, by Thermo Fisher Scientific (1 mentions) Av 400 spectrometer, by Bruker (1 mentions) E2695 separations module, by Waters Corporation (1 mentions) C18 column, by Agilent Technologies (1 mentions) Ultra performance liquid chromatography (uplc), by Waters Corporation (1 mentions) Acquity uplc beh c18, by Waters Corporation (1 mentions) Acquity ultra high performance liquid chromatographic system, by Waters Corporation (1 mentions) Poroshell ec c18 column, by Agilent Technologies (1 mentions)

9 protocols using sq detector

Enzymatic Synthesis of Ginsenoside C-Mc

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The minor ginsenoside C-Mc was prepared from American ginseng PPD ginsenosides, using 6% PPD ginsenoside substrate in acetate buffer (0.02 M and pH 5.0). Ginsenoside C-Mc was reacted with a volume of crude enzyme (A. niger g.848 strain) in the bioreactor.
In brief, chromatographic analysis was completed by a Waters ACQUITY UPLC system using XBridge C-18 chromatographic column (5 μm, φ4.6 × 250 mm) was used to analyze the samples. The mobile phase was acetonitrile (A) and water (B), column oven was 35°C, and flow rate is 0.6 ml/min. Detection wavelength of the DAD was 203 nm.
The mass examination was completed by a Waters SQ detector through a positive electrospray ionization pattern. Briefly, cone gas flow was set at 50 L/h, and desolvation gas flow was 550 L/h. Capillary voltage and cone voltage were 3.5 kV and 30 V, respectively.
The sample of ginsenoside C-Mc was identified as 20-O-[α-L-arabinofuranosyl-(1→6)-β-D-glucopyranosyl]-20(S)-protopanaxdiol using a BrukeAvance 600 (¹H: 600 MHz and ¹³C: 150 MHz) NMR spectrometer (Switzerland) in our previous study [42 (link)].

Liu X.Y., Li H., Hwang E., Park B., Xiao Y.K., Liu S., Fang J., Kim Y.J., Yi T.H, & Cai C. (2022). Chemical Distance Measurement and System Pharmacology Approach Uncover the Novel Protective Effects of Biotransformed Ginsenoside C-Mc against UVB-Irradiated Photoaging. Oxidative Medicine and Cellular Longevity, 2022, 4691576.

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UPLC-MS Quantification of Artemisinin

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Analysis of artemisinin was achieved by UPLC using a Waters Acquity UPLC™ system fitted with a single quadrupole (SQ) detector (Waters Corporation, Milford, MA). The mobile phase was an isocratic system consisting of 60% v/v aqueous solution of 0.1% formic acid and 40% v/v acetonitrile. The column was a Waters BEH300 C4 1.7 μm 100 mm × 2.1 mm column. The flow rate was 0.4 mL/min. For the four lowest concentration standards, a full loop (10.0 μL) of each sample was injected, and for the remaining standards 2.0 μL of the standard was injected using the partial loop with needle overfill injection mode.
Detection was achieved with a Waters SQ detector using selective ion monitoring of the [M-H₂O+H]⁺ with m/z of 265.3 in positive ion mode. The operation parameters were a capillary voltage of 3.5 kV, a cone voltage of 41 V, extractor of 3 V, and RF lens of 0.5 V. The source and desolvation temperatures were 135°C and 250° C, respectively. Pure 99.99% nitrogen was used at a flow rate of 500 L/hr for the desolvation and 2 L/hr for the cone. The operational parameters including the target mass were determined by running a mass scan and tuning the instrument to the desired mass.
The retention time was 2.2 minutes and quantification was based on the UPLC-MS peak area of artemisinin.

Graves R.A., Ledet G.A., Nation C.A., Showers P.R., Pramar Y.V., Mandal T.K, & Bostanian L.A. (2014). An Ultra High Pressure Liquid Chromatographic Method for the Determination of Artemisinin. Drug development and industrial pharmacy, 41(5), 819-824.

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Synthesis and Analytical Characterization of PACAP Peptides

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PACAP1-27 and PACAP1-38 were synthesized in our laboratory on a CEM Liberty microwave peptide synthesizer (Matthews, NC, USA) and were dissolved in the following sterile vehicles: (i) 0.9% saline solution, (ii) benzalkonium chloride solution for ophthalmic use (SOCB), (iii) thimerosal solution for ophthalmic use and (iv) water for injection, obtained from the Faculty Central Pharmacy, Faculty of Medicine, University of Szeged. A commercially available artificial tear solution (Systane Ultra^®, Alcon, Switzerland) was also used in the experiment.
Analytical reversed-phase high-performance liquid chromatography (RP-HPLC) was performed on an Agilent 1200 Series separation system with diode array and multiple wavelength detector (Waldbronn, Germany), with a Luna C18(2) 100 Å column (10 μm, 250 × 4.6 mm; Phenomenex, Torrance, CA, USA). The chromatography was carried out at room temperature (RT), with a flow rate maintained at 1.2 mL min⁻¹ at a wavelength of 220 nm [mobile phase solvent A: 0.1% TFA in Milli-Q water; solvent B: 0.1% TFA in acetonitrile (AcN)] using gradient elution. Mass spectrometry (MS) data were collected on a Waters SQ Detector (Milford, MA, USA) with an API mass spectrometer in positive ion mode.

Kovacs A.K., Atlasz T., Werling D., Szabo E., Reglodi D, & Toth G.K. (2020). Stability Test of PACAP in Eye Drops. Journal of Molecular Neuroscience, 71(8), 1567-1574.

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UPLC-MS Analysis of Photoreactive Compounds

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A2E (18 mM in DMSO) was diluted in DPBS to a final A2E concentration of 100 μM. Quercetin and cyanidin-3-glucoside were then added to obtain concentrations of 100 and 200 μM, and were irradiated (430 ± 30 nm, 1.5 milliwatts/cm²) for 3 min. The samples were examined by UPLC with a Waters SQ Detector (ion source; electrospray ionization mode (ESI), mass analyzer; single quadrupole). To acquire full scans, 5 μL of each sample was directly injected (without column) with a solvent of 70% acetonitrile/methanol (1:1) in 30% water containing 0.1% formic acid at a flow rate of 0.5 mL/min. UPLC-MS analysis was performed using positive ionization mode and voltages as follows: capillary voltage, 3.0 V; cone voltage, 30 V; extractor voltage, 3 V; and radio frequence lens voltage, 0.1 V. The desolvation gas flow was 800 liters/hr and the cone gas flow was 50 liters/hr; the source temperature was 150 °C, and desolvation temperature was 400 °C.

Wang Y., Kim H.J, & Sparrow J.R. (2017). Quercetin and cyanidin-3-glucoside protect against photooxidation and photodegradation of A2E in retinal pigment epithelial cells. Experimental eye research, 160, 45-55.

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Purification and Characterization of Cf Peptides

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The crude Cf peptides
were purified using a C18 RP-HPLC on a PerfectSil 100 ODS-3 5 μm
column (250 × 10 mm). All compounds are >95% pure by HPLC
analysis.
In most cases, the two regioisomers (5)-Cf and (6)-Cf were well separable
on the RP-HPLC column (100% purity of the given isomer confirmed by
NMR); only for ERD-B, an isomeric ratio of 80:20 could be achieved
during purification.
The mass accuracy of the products was determined
by ESI-MS using a Waters SQ detector (Milford, MA, USA). The mass
spectra were recorded in positive ion mode in the 200.0–3000.0 m/z range.

Sebák F., Horváth L.B., Kovács D., Szolomájer J., Tóth G.K., Babiczky Á., Bősze S, & Bodor A. (2021). Novel Lysine-Rich Delivery Peptides of Plant Origin ERD and Human S100: The Effect of Carboxyfluorescein Conjugation, Influence of Aromatic and Proline Residues, Cellular Internalization, and Penetration Ability. ACS Omega, 6(50), 34470-34484.

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Synthesis and Characterization of Diamidocalix[4]arene

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Solvents and reagents were obtained from commercial sources and used without further purification. Analytical TLC was performed using prepared plates of silica gel (Merck 60 Merck KGaA, Darmstadt, Germany F-254 on aluminum). ¹H and ¹³C NMR spectra were recorded on a Bruker Billerica, MA, USA AV400 spectrometer. All chemical shifts are reported in parts per million (ppm) using the residual peak of the deuterated solvent, whose values are referred to tetramethylsilane (TMS, δ_TMS = 0), as internal standard. ¹³C NMR spectra were performed with proton decoupling. Mass spectra were recorded in ESI mode on a single quadrupole instrument, SQ Detector, Waters (capillary voltage 3.7 kV, cone voltage 30–160 eV, extractor voltage 3 eV, source block temperature 80 °C, desolvation temperature 150 °C, and cone and desolvation gas (N₂) flow rates 1.6 and 8 L/min, respectively). UV-vis spectra were recorded on a Thermo Scientific Waltham, MA, USA Evolution 260 Bio spectrophotometer. Melting points were determined with a Gallenkamp apparatus.
Diamidocalix[4]arene 1 was synthesized according to a literature procedure [30 (link)].

Baldini L., Balestri D., Marchiò L, & Casnati A. (2023). A Combined Solution and Solid-State Study on the Tautomerism of an Azocalix[4]arene Chromoionophore. Molecules, 28(12), 4704.

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HPLC Separation and Analysis Protocol

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All HPLC separations involved a mobile phase of 0.05% (v/v) TFA in water (solvent A), and 0.04% (v/v) TFA in acetonitrile (solvent B).
Analytical HPLC–MS chromatographic separations were performed using a Waters Alliance e2695 Separations Module, an SQ Detector, and a Waters 2489 UV/Visible (UV/Vis) Detector equipped with an Agilent C18 column (5.0 μm, 4.6 × 150 mm) at a flow rate of 0.4 ml/min, or with a Welch-XB C4 column (3.0 μm, 3.0 × 150 mm) at a flow rate of 0.3 ml/min. The wavelengths of the UV detector were set to 210 and 220 nm.
Preparative HPLC separations were performed using a Hanbon Science & Technology NP7005C solvent delivery system and a Hanbon Science & Technology NU3010C UV detector equipped with an Exsil Pure 300 C18 column (10.0 μm, 20 × 250 mm) at a flow rate of 15 ml/min, or a Welch-XB C4 column (5 μm, 10 × 250 mm) at a flow rate of 4 ml/min. The wavelengths of UV-detector were set to 210 and 220 nm.

Wang Q., Yang X., Yuan R., Shen A., Wang P., Li H., Zhang J., Tian C., Jiang Z., Li W, & Dong S. (2024). A co-assembly platform engaging macrophage scavenger receptor A for lysosome-targeting protein degradation. Nature Communications, 15, 1663.

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UPLC-MS analysis of lipid families

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Samples were analysed by reverse phase UPLC–MS (UPLC, Waters, Acquity class H) coupled with a single quadrupole MS (SQDetector, Waters, Acquity) on an Acquity UPLC BEH C18 (Waters) 2.1 × 50 mm, 1.7 μm column. We used a method, based on acetonitrile gradients, that allowed the simultaneous detection of all three LP families. Elution was started at 30% acetonitrile (flow rate of 0.60 ml min⁻¹). After 2.43 min, the percentage of acetonitrile was brought up to 95% and held until 5.2 min. Then, the column was stabilized at an acetonitrile percentage of 30% for 1.7 min. Compounds were identified on the basis of their retention times compared with authentic standards (98% purity, Lipofabrik society, Villeneuve d'Asc, France) and the masses detected in the SQDetector. Ionization and source conditions were set as follows: source temperature, 130°C; desolvation temperature, 400°C; nitrogen flow, 1000 l h⁻¹; cone voltage, 120 V.

Cawoy H., Debois D., Franzil L., De Pauw E., Thonart P, & Ongena M. (2014). Lipopeptides as main ingredients for inhibition of fungal phytopathogens by Bacillus subtilis/amyloliquefaciens. Microbial Biotechnology, 8(2), 281-295.

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Quantitative LC-UV/MS Analysis of Compounds

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LC-UV/MS analysis was carried out on a Waters Acquity ultra-high performance liquid chromatographic system (Milford, MA, USA) equipped with a photodiode-array detector (PDA) and a single quadrupole mass spectrometer (Acquity SQ Detector) with an electrospray ionization source (ESI) operating in negative mode. An Agilent Poroshell EC-C18 column (150 mm × 4.6 mm, 2.7 µm) held at 50° C was used for the separation with 1 mM ammonium fluoride (NH4F) in water (solvent A) and acetonitrile (solvent B) as mobile phases. The flow rate was 0.6 mL/min with an elution gradient as follows: 0–2 min 75% A, 2–5 min 75–68% A, 5–14 min 68% A, 14–16 min 68–50% A, 16–18 min 50% A, 18–18.5 min 50–10% A, 18.5–22 min 10% A, 22–22.5 min 10–75% A, and 22.5–28 min 75% A. The injection volume was 5 µL. The mass acquisition window was from 50 to 2000 Da.

Yang Z., Uhler B., Zheng T, & Adams K.M. (2019). Enzymatic Synthesis and Characterization of a Novel α-1→6-Glucosyl Rebaudioside C Derivative Sweetener. Biomolecules, 9(1), 27.

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