Anti-DNP IgE, DNP-bovine serum albumin (BSA), dexamethasone, and Evans blue were purchased from Sigma (St. Louis, MO, USA). The RBL-2H3 cell line (KCLB-22256) was purchased from the Korean Cell Line Bank (Seoul, Korea). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum, and penicillin-streptomycin (PS) were purchased from Hyclone (Logan, UT, USA). The histamine, TNF-α, IL-6, IL-1β, and IL-4 ELISA kits were purchased from Biovision (Milpitas, CA, USA). Anti-phospho-p38 (Thr180/Tyr182), anti-phospho-ERK (L352), anti-phospho-JNK (T183/Y185), anti-p38, anti-ERK, anti-JNK, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Boehmeria nivea is an eco-friendly pesticide-free product, which was purchased from a farming association (Yeonggwang, Korea).
Histamine
Manufactured by Abcam
Sourced in United States
Histamine is a laboratory reagent that is used as a standard in various biochemical and immunological assays. It is a small organic molecule that plays a role in several physiological processes. Histamine is commonly used as a reference compound in the quantification and analysis of histamine levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Anti dnp ige, by Merck Group (1 mentions)
Tnf α, by Abcam (1 mentions)
Interleukin 6 (il 6), by Abcam (1 mentions)
Anti erk, by Santa Cruz Biotechnology (1 mentions)
Anti jnk, by Santa Cruz Biotechnology (1 mentions)
Dnp bovine serum albumin, by Merck Group (1 mentions)
Il 1β, by Abcam (1 mentions)
Evans blue, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Total immunoglobulin e ige, by BD (1 mentions)
Interferon γ ifn γ, by BioLegend (1 mentions)
Interleukin 4 (il 4), by BioLegend (1 mentions)
Protease inhibitor, by Roche (1 mentions)
2 protocols using histamine
1
Boehmeria nivea Extract Inhibits Mast Cell Activation
2
Immunological Markers in CO2-Asphyxiated Mice
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Blood samples were collected from the hearts of CO2-asphyxiated mice and were centrifuged (10,000g, 10 min) to obtain serum. The total protein was isolated from 100 mg of dorsal skin tissue by homogenization in 1 mL of a tissue protein extraction reagent (Pierce, Rockford, IL, USA) with protease inhibitors (Roche, Indianapolis, IN, USA) and was quantified by a bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA). The levels of total immunoglobulin E (IgE) (BD Pharmingen, San Diego, CA, USA), histamine (BioVision, Milpitas, CA, USA), interleukin (IL)-6, IL-17A (eBioscience, San Diego, CA, USA), IL-4, interferon-γ (IFN-γ) (BioLegend, San Diego, CA, USA), and thymic stromal lymphopoietin (TSLP; Abcam, Cambridge, MA, USA) were analyzed by sandwich ELISA (enzyme-linked immunosorbent assay) according to the manufacturer’s instructions.
The spleens were weighed. The splenic weight index was defined as the spleen weight divided by the whole body weight.
The spleens were weighed. The splenic weight index was defined as the spleen weight divided by the whole body weight.
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