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Balb c

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BALB/c is a strain of laboratory mice commonly used in scientific research. It is an inbred mouse strain that is widely used as a model organism for various studies, including immunology, infectious diseases, and cancer research.

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Lab products found in correlation

C57bl 6, by Taconic Biosciences (9 mentions) Ncr nu nu, by Taconic Biosciences (3 mentions) C57bl 6, by Jackson ImmunoResearch (3 mentions) Balb c mice, by Jackson ImmunoResearch (2 mentions) Streptavidin alexa fluor 546, by Thermo Fisher Scientific (2 mentions) Anti cd44 fitc, by Thermo Fisher Scientific (2 mentions) Dapi and anti hif1α fluorescein, by R&D Systems (2 mentions) 1ar ultra fast spectral scanning confocal microscope, by Nikon (2 mentions) Nod scid mice, by Jackson ImmunoResearch (2 mentions) Athymic nu nu, by Taconic Biosciences (1 mentions) Vevo 2100, by Fujifilm (1 mentions) Fvb n, by Taconic Biosciences (1 mentions) D luciferin, by PerkinElmer (1 mentions) B6 sjl, by Taconic Biosciences (1 mentions) B6.129p2 sjl myd88tm1defr j myd88fl fl, by Jackson ImmunoResearch (1 mentions) C57bl 6 b6, by Jackson ImmunoResearch (1 mentions) C57bl 6j, by Taconic Biosciences (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions) Dba 2, by Jackson ImmunoResearch (1 mentions) Nod mice, by Taconic Biosciences (1 mentions)

29 protocols using balb c

1

Isolation and Transfer of TREG Cells

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BALB/c (Taconic) or β2-AR−/− BALB/c (Taconic) mice were sacrificed to harvest spleen and lymph nodes for CD4+CD25+ TREG cells. Organs were homogenized and red blood cells lysed to yield a single cell suspension. The concentrations of cells were adjusted for 5 × 105CD4+CD25+ TREG cells in 300 μL of no serum Dulbecco’s Modified Eagle’s Medium (DMEM). Each wildtype BALB/c recipient mouse received cells via intravenous (i.v.) injection in the tail vein while in a mouse restrainer on Day − 1 of the OVA-sensitization and challenge protocol (see Fig. 1*) [21 (link)].
Dugger K.J., Chrisman T., Sayner S.L., Chastain P., Watson K, & Estes R. (2018). Beta-2 adrenergic receptors increase TREG cell suppression in an OVA-induced allergic asthma mouse model when mice are moderate aerobically exercised. BMC Immunology, 19, 9.
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2

Immune Response of Young and Aged BALB/c Mice to SARS-CoV-2 Vaccines

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Female 6–8 weeks old young adult BALB/c mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). Aged (15 months old) BALB/c mice from National Cancer Institute (NCI/NIH, Bethesda, MD, USA) or retired 8 to 9 months (M) old BALB/c mice were obtained from Taconic Farms (Hudson, NY, USA) and aged to become 15 M old. Animal experiments were performed under the guidelines of the approved institutional animal care and use committee (IACUC) protocol (A21004). Young adult or 15 M old aged BALB/c mice (n = 5–6 per group) were intramuscularly (IM) immunized twice (or three times) at 3- or 4-week intervals with full-length S (S1–S2, 0.8 µg equal to 5.9 nanomoles (nM) in young and aged mice, 4 µg equal to 29.8 nM in aged mice), S1 (0.8 µg 10.5 nM or 4 µg 12.3 nM) and S2 (4 µg equal to 67.4 nM), and inactivated SARS-CoV-2 (0.8 µg prime and 10 µg boost). Adjuvants (MPL + QS-21, 1 µg + 10 µg, respectively) were included in S, S1, S2, and inactivated SARS-CoV-2 vaccination as indicated. Blood samples were collected 2 or 3 weeks after immunization to determine IgG binding and neutralizing antibodies.
Kim K.H., Bhatnagar N., Jeeva S., Oh J., Park B.R., Shin C.H., Wang B.Z, & Kang S.M. (2021). Immunogenicity and Neutralizing Activity Comparison of SARS-CoV-2 Spike Full-Length and Subunit Domain Proteins in Young Adult and Old-Aged Mice. Vaccines, 9(4), 316.
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3

In Vivo Nanoparticle Biodistribution

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A total of 5 × 106 MDA-MB-231 cells (1:1 PBS:Matrigel) were injected subcutaneously into the hindflanks of nude mice (NCR nu/nu, Taconic). Tumors were allowed to form over 3 to 4 weeks, after which treatment groups were randomized. Nanoparticles (8.3 × 1012 NP/kg, 5%glucose) were injected via the tail vein into tumor-bearing nude or immunocompetent mice (BALB/c, Taconic). Whole-animal imaging was performed using a Xenogen IVIS Imaging System (Caliper) and 100-nm fluorescent carboxylate-modified polystyrene beads (Life Technologies; Infrared 715/755) as surrogates for dextran sulfate-conjugated liposomes. Free drugs were dosed o.g. at nanoparticle-equivalent drug concentrations (5% d-glucose, 1% polysorbate 80). Albumin, blood urea nitrogen (BUN), and creatinine (Cr) levels were measured by Charles River Laboratories from serum samples obtained via cardiac puncture 24 hours following nanoparticle (i.v.) or free drug (o.g.) administration in 6- to 8-week-old female BALC/c mice. These experiments were approved by the Massachusetts Institute of Technology Committee on Animal Care (CAC).
Dreaden E.C., Kong Y.W., Morton S.W., Correa S., Choi K.Y., Shopsowitz K.E., Renggli K., Drapkin R., Yaffe M.B, & Hammond P.T. (2015). Tumor-Targeted Synergistic Blockade of MAPK and PI3K from a Layer-by-Layer Nanoparticle. Clinical cancer research : an official journal of the American Association for Cancer Research, 21(19), 4410-4419.
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4

In Vivo Tumor Growth Evaluation

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Male athymic nu/nu, Balb/C, and DBA/2 mice, 5–6 weeks of age, were purchased from Taconic and handled in accordance with approved Institutional Animal Care and Use Committee protocols. Following acclimation, mice were inoculated with A549/HAS3, AsPC-1, AsPC-1/HAS3, BxPC-3, BxPC-3/HAS3, H2170/HAS3, WT-CLS1/HAS3 (5 × 106, athymic nu/nu), or CT26/HAS3 cells (2 × 105, Balb/C) adjacent to the right tibia periosteum. KLN205 cells were inoculated s.c. into the right flank of DBA/2 mice (31 (link), 33 (link)). Tumor growth in peritibial models was determined by acquiring three-dimensional (3D) tumor images twice weekly using a high-resolution ultrasound imaging system (Vevo 2100; FUJIFILM VisualSonics), and subsequently the associated 3D tumor volume software was used to determine tumor volume. Subcutaneous tumors were measured using an electronic caliper (Vernier Software & Technology) and tumor volume in mm3 calculated using the formula: Tumor volume = 1/2[length × (width)2].
Li X., Shepard M.H., Cowell J.A., Zhao C., Osgood R.J., Rosengren S., Blouw B., Garrovillo S.A., Pagel M.D., Whatcott C.J., Han H., Von Hoff D.D., Taverna D.M., LaBarre M.J., Maneval D.C, & Thompson C.B. (2018). Parallel Accumulation of Tumor Hyaluronan, Collagen, and Other Drivers of Tumor Progression. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(19), 4798-4807.
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5

BALB/c Mouse Model for In Vitro and Adoptive Transfer

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Six to eight week-old female BALB/c (Taconic) were used for all recipient and wild type experiments. Age-matched β2-AR−/− BALB/c (from Dr. Virginia Sanders, Ohio State University managed Taconic colony, with permission from Dr. Kobilka, ( [19 (link), 20 (link)])) were used for in vitro and adoptive transfer (ADTX) experiments as indicated. All mice were maintained in autoclaved microisolator cages (Lab Products) and provided with food (Teklad) and water, as needed. All mice were housed in the University of South Alabama, College of Medicine, Animal Care Facility. At the end of each protocol, mice were terminated using 1) overdose of chemical anesthetics (ketamine 80 mg/kg:Xylazine 10 mg/kg-- 2-3 times the anesthetic dose) to loss of toe pinch and corneal reflex and, either 2) exsanguination by intracardiac puncture or 3) vital tissue/organ collection (removal of lungs) as per IACUC approval (USA - IACUC 346362–4).
Dugger K.J., Chrisman T., Sayner S.L., Chastain P., Watson K, & Estes R. (2018). Beta-2 adrenergic receptors increase TREG cell suppression in an OVA-induced allergic asthma mouse model when mice are moderate aerobically exercised. BMC Immunology, 19, 9.
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6

Mouse Breeding and Pathogen-Free Maintenance

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C57BL/6, FVB/N, and BALB/c mice were purchased from Taconic Farms. All animals used in this study were males, 8–10 weeks of age. Animals were bred and maintained under specific pathogen‐free (SPF) conditions in Thoren Isolator racks under positive pressure. The Institutional Care and Use Committee of the University of Alabama at Birmingham (UAB) approved all experiments. SPF conditions at UAB include an absence of the following organisms, as determined by serological screening: mouse parvoviruses, including MPV‐1, MPV‐2, and minute virus of mice; mouse hepatitis virus, murine norovirus, Theiler's murine encephalomyelitis virus; mouse rotavirus (epizootic diarrhea of infant mice), Sendai virus; pneumonia virus of mice; reovirus; Mycoplasma pulmonis; lymphocytic choriomeningitis virus; mouse adenovirus; ectromelia (mousepox) virus; K polyomavirus; and mouse polyomavirus. Testing and other methods were as described at http://main.uab.edu/Sites/ComparativePathology/surveillance/.
Tanner S.M, & Lorenz R.G. (2022). FVB/N mouse strain regulatory T cells differ in phenotype and function from the C57BL/6 and BALB/C strains. FASEB BioAdvances, 4(10), 648-661.
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7

In Vivo Nanoparticle Tumor Targeting

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In total, 5 ×106 A549, MDA-MB-468, or MDA-MB-468/Luc cells (1:1 PBS:Matrigel) were injected subcutaneously into the hindflanks of nude mice (NCR nu/nu, Taconic). Tumors were allowed to form for 2–3 weeks. Nanoparticles (8.3 × 1012 NP·kg–1, 5% glucose) were injected via the tail vein into tumor-bearing nude or immunocompetent mice (BALB/c, Taconic). Tumors were harvested after 48 or 72 h and processed by the Swanson Biotechnology Histology Core Facility. Briefly, tumors were formalin-fixed, paraffin-embedded, sectioned (5 μm), deparaffinized, antigen retrieved, and stained using DAPI, biotinylated hyaluronic acid binding protein (Calbiochem), anti-CD44-FITC (Life Technologies), and streptavidin-Alexa Fluor 546 (Life Technologies) or DAPI and anti-HIF1α-fluorescein (R&D Systems). Slides were mounted and imaged using a Nikon 1AR Ultra-Fast Spectral Scanning Confocal Microscope. Whole-animal imaging was performed using a Xenogen IVIS Imaging System (Caliper) with d-luciferin (150 mg kg–1 ip, PerkinElmer) as a bioluminescent substrate. These experiments were approved by the Massachusetts Institute of Technology Committee on Animal Care (CAC).
Dreaden E.C., Morton S.W., Shopsowitz K.E., Choi J.H., Deng Z.J., Cho N.J, & Hammond P.T. (2014). Bimodal Tumor-Targeting from Microenvironment Responsive Hyaluronan Layer-by-Layer (LbL) Nanoparticles. ACS Nano, 8(8), 8374-8382.
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8

Generation of IL-4Rα Conditional Knockout Mice

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IL-4Rαflox/Δ LysMWT/Cre mice backcrossed on a BALB/c background were kindly provided by Dr. Fred Finkelman (U. Cincinnati, Ohio) and Dr. Frank Brombacher (University of Cape Town; Cape Town, South Africa) [7] (link). IL-4Rαflox/flox females were crossed with IL-4RαΔ/Δ LysMWT/Cre males to generate IL-4Rαflox/Δ LysMWT/Cre mice (called IL-4Rαflox/ΔLysMCre in this paper) and Cre-negative IL-4Rαflox/Δ littermates. All cells in both the Cre-positive and Cre-negative mice maintain the Il4rα gene on one allele. This breeding scheme prevents embryonic deletion of IL-4Rα by Cre-expressing females. BALB/c and IL-4RαΔ/Δ mice were obtained from Taconic Farms Inc (Derwood, MD). All animals were housed under specific pathogen-free conditions at the National Institutes of Health in an American Association for the Accreditation of Laboratory Animal Care-approved facility.
Vannella K.M., Barron L., Borthwick L.A., Kindrachuk K.N., Narasimhan P.B., Hart K.M., Thompson R.W., White S., Cheever A.W., Ramalingam T.R, & Wynn T.A. (2014). Incomplete Deletion of IL-4Rα by LysMCre Reveals Distinct Subsets of M2 Macrophages Controlling Inflammation and Fibrosis in Chronic Schistosomiasis. PLoS Pathogens, 10(9), e1004372.
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9

Generation and Maintenance of Transgenic Mouse Lines

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IL-12Rβ2fl/fl mice were generated at the Brown Transgenic Facility, as previously described (21 (link)). B6.129P2(SJL)-MyD88tm1Defr/J (MyD88fl/fl, Cat #: 008888), B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J (eYFP, Cat #: 006148), IL-18−/− (Cat #: 004130), and C57BL/6 (B6, Cat #: 000664) mice were purchased from Jackson Laboratory. B6.Cg-Tg(CD4-cre)1Cwi N9 (CD4cre, Cat #: 4196), Balb/c (Cat #: BALB-F), and B6.SJL (Cat #: 002014) mice were purchased from Taconic. IL-12Rβ2fl/fl CD4cre+/− and MyD88fl/fl eYFP+/− CD4cre+/− mice were generated and maintained in-house, along with littermate controls (IL-12Rβ2fl/+ CD4cre+/− and MyD88fl/+ eYFP+/− CD4cre+/−, respectively). Both age- and sex-matched female and male mice (6–26 weeks) were used for these studies; littermates were used as controls for MyD88 cKO and IL-12βR2 cKO mice. All experiments were performed in accordance to the Guide for the Care of Use of Laboratory Animals, as defined by the NIH (PHS Assurance #A3284–01). The Institutional Animal Care and Use Committee (IACUC) of Brown University reviewed, and approved, the animal protocols performed in this study. Animals were housed in an AAALAC-accredited and centralized research facility.
Anderson C.K., Reilly S.P, & Brossay L. (2020). The iNKT cell response has differential signaling requirements during Ag-dependent and Ag-independent activation. Journal of immunology (Baltimore, Md. : 1950), 206(1), 132-140.
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10

Generation and Genotyping of Knockout Mice

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BALB/c and C57BL/6J mice were purchased from Taconic Biosciences and Jackson laboratory (Bar Harbor, ME), respectively. Mating pairs of Cybb-/- in C57BL/6J background, and Skap2+/- (B6.129S5-Skap2Gt(VICTR20)21Lex/Mmjax) were purchased from Jackson laboratory and bred at Tufts University in the specific pathogen-free facility of Tufts University. Generation of Skap2-/- mice in the BALB/c background was previously described (Togni et al., 2005 (link); Alenghat et al., 2012 (link)). Skap2-/- mice were genotyped by two independent PCRs using primer sequences: SkapLeft-forward primer (common for both PCRs), 5’ CAG CTT GCC GAC TTT TCT; GTLexVir, 5’GAG GGC TGG ACC GCA TCT GG; GTSkapRight, 5’CCG CCT CCC ACC CCT CAA TC following procedures described from Jackson laboratory and (Alenghat et al., 2012 (link)). All mice were handled in accordance with protocols approved by the Institutional Animal Care and Use Committee of Tufts University.
Nguyen G.T., Shaban L., Mack M., Swanson K.D., Bunnell S.C., Sykes D.B, & Mecsas J. (2020). SKAP2 is required for defense against K. pneumoniae infection and neutrophil respiratory burst. eLife, 9, e56656.
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