Fluorescein isothiocyanate fitc labeled dextran

Antibody-sensitized sheep erythrocytes (EA), rabbit erythrocytes, normal human serum (NHS), C5-depleted or C9-depleted human serum, human complement proteins (including C5, C6, C7, C8, C9, and C5b6), and gelatin veronal buffer (GVB) and gelatin veronal buffer with calcium and magnesium (GVB++) were purchased from Complement Technology, Inc. (Tyler, TX, USA). Human C9 protein was conjugated with Alexa Fluor 647 dye using a labeling kit (Invitrogen, Carlsbad, CA, USA). Zilucoplan was prepared internally (UCB Ra Pharmaceuticals, Cambridge, MA, USA). eculizumab biosimilar was purchased from Syd Labs Inc. (Hopkinton, MA, USA). Labeling of eculizumab biosimilar with Alexa Fluor™ 488 NHS Ester (AF488) (Thermo Fisher, Waltham, MA, USA) was conducted using a labeling kit and following the vendor’s protocol (Supplementary Material). Fluorescein isothiocyanate (FITC)-labeled dextran (molecular weight approximately 4,000 and 150,000 Da; FITC-dextran 4 kDa and FITC-dextran 150 kDa, respectively) was from Chondrex (Woodinville, WA, USA).
Animal sera used in cross-species activity assay included rat serum (Complement Technology) and sera of baboon, beagle, chimpanzee, cynomolgus monkey, guinea pig, minipig, mouse, pig, rhesus monkey, and rabbit (all from BioIVT, Hicksville, NY, USA).

Antibody-sensitized sheep erythrocytes (EA), rabbit erythrocytes, normal human serum (NHS), C5-depleted or C9-depleted human serum, human complement proteins (including C5, C6, C7, C8, C9, and C5b6), and gelatin veronal buffer (GVB) and gelatin veronal buffer with calcium and magnesium (GVB++) were purchased from Complement Technology, Inc. (Tyler, TX, USA). Human C9 protein was conjugated with Alexa Fluor 647 dye using a labeling kit (Invitrogen, Carlsbad, CA, USA). Zilucoplan was prepared internally (UCB Ra Pharmaceuticals, Cambridge, MA, USA). eculizumab biosimilar was purchased from Syd Labs Inc. (Hopkinton, MA, USA). Labeling of eculizumab biosimilar with Alexa Fluor™ 488 NHS Ester (AF488) (Thermo Fisher, Waltham, MA, USA) was conducted using a labeling kit and following the vendor’s protocol (Supplementary Material). Fluorescein isothiocyanate (FITC)-labeled dextran (molecular weight approximately 4,000 and 150,000 Da; FITC-dextran 4 kDa and FITC-dextran 150 kDa, respectively) was from Chondrex (Woodinville, WA, USA).
Animal sera used in cross-species activity assay included rat serum (Complement Technology) and sera of baboon, beagle, chimpanzee, cynomolgus monkey, guinea pig, minipig, mouse, pig, rhesus monkey, and rabbit (all from BioIVT, Hicksville, NY, USA).