Dioctyl sulfosuccinate sodium salt

We suspend Nile Red–dyed PMMA colloids and polyhydroxystearic acid polymer–grafted PMMA colloids in a mixture of cis-decahydronaphthalene and tetrachloroethylene (Sigma-Aldrich), whose refractive index and density are closely matched with those of particles, inside a glass capillary. The PMMA particles have diameter σ = 1.8 μm, with the polydispersity of less than 2.5%. The colloidal particle volume fraction ϕ is around 54%. We find that adding 50 mM/liter dioctyl sulfosuccinate sodium salt (Sigma-Aldrich) to the system largely screens surface charges on particles, which allows us to regard the system as hard-sphere like. The other experimental procedures are the same as the charged colloidal systems.

Poly(vinyl alcohol) (PVA; M_W = 30,000 g·mol⁻¹; 100% hydrolyzed,), poly(ϵ-caprolactone) (PCL; M_W = 80,000 g·mol⁻¹), 2-(N-morpholino)ethanesulfonic acid (MES), 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS), phosphate-buffered saline (PBS), succinic anhydride, dioctyl sulfosuccinate sodium salt (AOT), and ciprofloxacin (CF) were purchased from Sigma Aldrich, Steinheim, Germany. Gentamicin sulphate, 4-Dimethylaminopyridine (4-DMAP), ethanol, 2-propanol, acetone, and dichloromethane (DCM) were purchased from Roth (Karlsuhe, Germany). d-(Aspartic acid)₆ oligomers (ASP) were synthesized on demand by GenScript (Leiden, The Netherlands). Tryptin Soy Agar (TSA) plates were purchased from Oxoid AG (Basel, Switzerland). CellTiter Blue^® cell viability assay was purchased from Promega, Madison, WI, USA. Bone marrow-derived mesenchymal stem cells (BMSC) were isolated from human bone marrow donated by the Inselspital Bern. Osteoassay plates were acquired from Corning (Amsterdam, The Netherlands). The bacterial strains used were methicillin susceptible S. aureus JAR 890 (CCOS, Wädenswill, Switzerland) and E. coli O76:H51 (CCOS, Wädenswill, Switzerland).

The organic solvents heptane, toluene, xylene, and cyclohexane were purchased from Romical, along with chemicals and laboratory equipment. Poly(1-vinylpyrrolidone)-graft-(1-hexadecene) co-polymer (containing 80% by weight of C₁₆ α-olefin and 20% by weight of polymerized vinylpyrrolidone) (Agrimer AL-22) was purchased from International Specialty Products (ISP). Bis-poly(ethylene glycol)/poly(propylene glycol)-14/14 dimethicone (ABIL EM 97) was purchased from Evonik. Poly(ethylene glycol) 200, sodium tetrachloropalladate (Na₂PdCl₄), dioctyl sulfosuccinate sodium salt (AOT), sorbitan monooleate (span 80), poly(ethylene glycol) oleyl ether (brij 92v), branched poly(ethylene amine) 800, 2,2′(ethylenedioxy)bis(ethylamine), hexamethylenediamine (HMDA), and diethylenetriamine (DETA) were purchased from Sigma Aldrich-Merck. Polymethylene polyphenyl isocyanate (PAPI 27) was contributed by FMC Corporation. Toluene-2,4-diisocyanate (TDI) was purchased from Acros Organics. All substrates were purchased either from Alfa Aesar (Tewksbury, MA, USA) or Sigma Aldrich-Merck (Budapest, Hungary).

HPLC grade water and LC-MS grade methanal were purchased from Acros Organics (New Jersey). Lysophosphatidylcholine 17:1 (LPC 17:1) was purchased from Avanti Polar Lipids (Alabaster, AL). The extraction solvent used in this study was composed of 1 μM LPC 17:1 in 9 : 1 MeOH : H₂O (v/v). Fused silica capillaries (50 μm ID, 150 μm OD) were purchased from Polymicro Technologies (Phoenix, AZ). The MinuteWeld epoxy glue was purchased from J-B Weld company (Sulphur Springs, TX). A 2000 grit silicon carbide sandpaper (STARCKE, Germany) was used to polish the SLE-MFP sampling port.
4′′ fused-silica substrates with a thickness of 1 mm were obtained from Siegert Wafer GmbH (Aachen, Germany). Potassium hydroxide solution (45%, extra pure) and MeOH (HPLC grade) were purchased from Carl Roth GmbH & Co. KG (Karlsruhe, Germany). Dioctyl sulfosuccinate sodium salt (≥99%) was purchased from Sigma-Aldrich GmbH (Taufkirchen, Germany).

All chemicals were used
as received unless stated otherwise: Zonyl FS-300 (40% solids in water,
ABCR), sodium dodecyl sulfate (99%, Sigma-Aldrich), 1H,1H,2H,2H-perfluorodecyl
acrylate (97%, ABCR), 1,6 hexanediol diacrylate (80%, Sigma-Aldrich),
2-hydroxy-2-methyl-propiophenone (97%, Sigma-Aldrich), 4-dimethylaminopyridin
(99%, Sigma-Aldrich), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (98%, Sigma-Aldrich),
1-hydroxybenzotriazole hydrate (99%, Sigma-Aldrich), fluoresceinamine
(Sigma-Aldrich), 1-octanol (99%, Sigma), gold(III) chloride trihydrate
(49 wt % Au Sigma-Aldrich), sodium citrate, sodium borohydride, 10-hydroxydecanoic
acid (technical grade, Sigma-Aldrich), trimethylamine (99%, Sigma-Aldrich),
acryloyl chloride (97%, Sigma-Aldrich), dichloromethane (Sigma-Aldrich),
Pluronic F-127 (Sigma-Aldrich), dioctyl sulfosuccinate sodium salt
(Sigma-Aldrich), trimethylolpropane ethoxylate triacrylate (∼428
Mn, Sigma-Aldrich), and d-glucose (Carl Roth). Deionized
water was used as the emulsion continuous phase. 10-(Acryloyloxy)decanoic
acid (ADA) was synthesized using a modified literature procedure (see
the Supporting Information for details).^{49 (link)} Gold nanoparticles were freshly prepared according
to a known literature procedure.^{50 (link)}