Total RNA was reverse-transcribed into complementary DNA (cDNA) using the RT First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland). Quantitative real-time PCR (qRT-PCR) was performed using 2 × SYBR Green qPCR Master Mix (Servicebio, Wuhan, China) by an ABI QuantStudio 5 PCR machine (Applied Biosystems; Thermo, Waltham, MA, USA) with the following programs: 1 cycle at 95 °C for 10 min, 40 cycles at 95 °C for 15 s and 60 °C for 30 s. The primer sequences are provided in Table 2 . Relative mRNA levels of specific genes were quantified using the 2−ΔΔCt method values with β-actin as the reference gene.
Rt first strand cdna synthesis kit
Manufactured by Roche
Sourced in Switzerland, Germany
The RT First Strand cDNA Synthesis Kit is a reagent kit used for the reverse transcription of RNA into complementary DNA (cDNA). It contains the necessary components to perform this process, including a reverse transcriptase enzyme, buffer solutions, and other relevant reagents.
Automatically generated - may contain errors
2 protocols using rt first strand cdna synthesis kit
1
Quantitative RT-PCR Analysis of Gene Expression
2
RNA Extraction and qRT-PCR Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted with RNAiso Plus Reagent (TransGen Biotech, China) and reverse-transcribed into complementary DNA (cDNA) using the RT First Strand cDNA Synthesis Kit (Roche, Germany). Quantitative real-time PCR (qRT-PCR) was performed using an ABI QuantStudio 5 PCR machine (Applied Biosystems; Thermo, Waltham, MA, USA) at 95 °C for 10 min, followed by 40 cycles of 95 °C for 15 s and 60 °C for 30 s, with 2×SYBR Green qPCR Master Mix (Servicebio, Wuhan, China). The primer sequences are provided in Table 3 . Relative mRNA levels of specific genes were quantified using the 2−ΔΔCt method values with respect to the values for β-actin.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!