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Isre reporter pgl4

Manufactured by Promega
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Sourced in United States

The ISRE reporter pGL4.45 is a plasmid-based reporter system designed to monitor the activity of the interferon-stimulated response element (ISRE) in mammalian cells. It contains the ISRE sequence upstream of a minimal promoter that drives the expression of the firefly luciferase gene. This reporter system can be used to study the transcriptional regulation of genes that are responsive to type I interferon signaling.

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Lab products found in correlation

Minimal promoter pgl4, by Promega (2 mentions) Nano glo system, by Agilent Technologies (2 mentions) Cytation5 plate, by Agilent Technologies (2 mentions) Poly 1 c, by Merck Group (2 mentions)

2 protocols using isre reporter pgl4

1

Evaluating NS1 Protein Binding Regions

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Luciferase reporter assays were performed with 2.5×104 293T cells per well of a 96-well plate transfected with pNL1.1.TK (control NanoLuc vector, Promega, Madison, WI, USA), plus WT or Y125A mutant NS1 or empty vector (pCAGGS), with or without 4 μg/mL poly(I:C) (Sigma), plus luciferase reporter plasmids: minimal promoter pGL4.23 (Promega) or ISRE reporter pGL4.45 (Promega) or minimal promoter pGL4.23 (Promega) containing one of the NS1 binding regions. Luciferase activity was measured 24 h post-transfection (triplicate wells per condition, 96-well plate) using the Promega Nano-Glo system per manufacturer protocol in a BioTek Cytation5 plate reader. All experiments were performed at least twice.
Pei J., Beri N.R., Zou A.J., Hubel P., Dorando H.K., Bergant V., Andrews R.D., Pan J., Andrews J.M., Sheehan K.C., Pichlmair A., Amarasinghe G.K., Brody S.L., Payton J.E, & Leung D.W. (2021). Nuclear-localized human respiratory syncytial virus NS1 protein modulates host gene transcription. Cell reports, 37(2), 109803.
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2

Evaluating NS1 Protein Binding Regions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Luciferase reporter assays were performed with 2.5×104 293T cells per well of a 96-well plate transfected with pNL1.1.TK (control NanoLuc vector, Promega, Madison, WI, USA), plus WT or Y125A mutant NS1 or empty vector (pCAGGS), with or without 4 μg/mL poly(I:C) (Sigma), plus luciferase reporter plasmids: minimal promoter pGL4.23 (Promega) or ISRE reporter pGL4.45 (Promega) or minimal promoter pGL4.23 (Promega) containing one of the NS1 binding regions. Luciferase activity was measured 24 h post-transfection (triplicate wells per condition, 96-well plate) using the Promega Nano-Glo system per manufacturer protocol in a BioTek Cytation5 plate reader. All experiments were performed at least twice.
Pei J., Beri N.R., Zou A.J., Hubel P., Dorando H.K., Bergant V., Andrews R.D., Pan J., Andrews J.M., Sheehan K.C., Pichlmair A., Amarasinghe G.K., Brody S.L., Payton J.E, & Leung D.W. (2021). Nuclear-localized human respiratory syncytial virus NS1 protein modulates host gene transcription. Cell reports, 37(2), 109803.
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