Luminol

Manufactured by Biosynth

Luminol is a chemiluminescent compound that emits light when oxidized. It is commonly used in forensic investigations to detect the presence of blood at crime scenes, as it can reveal trace amounts of blood that are not visible to the naked eye. Luminol reacts with the iron in hemoglobin, the oxygen-carrying protein in blood, to produce a blue-green glow that can be photographed and analyzed.

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Lab products found in correlation

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Spelling variants of this product

Luminol Sodium Salt (2 citations)

3 protocols using luminol

Flow Cytometry Analysis of Immune Cells

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Stained cells were analyzed using a BD LSR II-SORP system (Becton Dickinson) and analyzed with FlowJo software (Tree Star). The following mAbs were used: CD45-PerCPCy5, CD45-APC, CD8-APC, anti-IFNγ-PECy7 and anti-IL-4-FITC (BD Biosciences), Gr1-APC, CD11c-PECy7, CD11b-PB, F4/80-APC (eBiosciences), 1A8-APCCy7, Ly6C-FITC, 1A8-APCCy7, CD4-AF700. DAPI and AnV-FITC (BioLegends). In vivo bioluminescence imaging of MPO activity was quantified through injection of 200mg/kg luminol (Carbosynth) i.p. 10 minutes before luminescence acquisition. Photon emission was acquired for 10 minutes using a Xenogen IVIS Imaging system.

Hurrell B.P., Schuster S., Grün E., Coutaz M., Williams R.A., Held W., Malissen B., Malissen M., Yousefi S., Simon H.U., Müller A.J, & Tacchini-Cottier F. (2015). Rapid Sequestration of Leishmania mexicana by Neutrophils Contributes to the Development of Chronic Lesion. PLoS Pathogens, 11(5), e1004929.

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Labeling and Tracking Leishmania Major

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L. major LV39 mCherry parasites were generated by transfecting log-stage promastigotes with linearized mCherry expression plasmid using the AMAXA nucleofection system (Lonza, Basel, Switzerland) and selecting clones with 50 μg/ml hygromycin B (Calbiochem Merck, Darmstadt, Germany), as detailed in the Supplementary Materials. In selected experiments, L. major was labeled with 5 μmol/L CMFDA according to the manufacturer’s protocol (Molecular Probes). Reactive oxygen species formation was measured with the dihydrodamine 123 probe (Sigma-Aldrich, St. Louis, MO) or luminol (Carbosynth, Newbury, UK) as described by the manufacturers.

Ronet C., Passelli K., Charmoy M., Scarpellino L., Myburgh E., La Torre Y.H., Turco S., Mottram J.C., Fasel N., Luther S.A., Beverley S.M., Launois P, & Tacchini-Cottier F. (2018). TLR2 Signaling in Skin Nonhematopoietic Cells Induces Early Neutrophil Recruitment in Response to Leishmania major Infection. The Journal of investigative dermatology, 139(6), 1318-1328.

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Neutrophil ROS Production Assay

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ROS production was measured using a Luminol-based chemiluminescence assay. 5 × 10⁵ neutrophils were incubated with either L. (V.) p., PMA or without stimulus in white, opaque 96-well plates (Perkin Elmer). Luminol (Carbosynth) was added at a final concentration of 20 μg/mL. ROS induced chemiluminescence was measured at all wavelengths with a plate reader at 5 min intervals during 1 h.

Regli I.B., Fernández O.L., Martínez-Salazar B., Gómez M.A., Saravia N.G, & Tacchini-Cottier F. (2018). Resistance of Leishmania (Viannia) Panamensis to Meglumine Antimoniate or Miltefosine Modulates Neutrophil Effector Functions. Frontiers in Immunology, 9, 3040.

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