The lung, spleen, brain, liver, kidney and testis of each rat were digested in the microwave digestion instrument by adding 2 mL 65% nitric acid (HNO3). The digested samples were diluted to 10 mL using ultra-pure water. The indium concentration in tissues was determined using an inductively coupled plasma mass spectrometer (ICP-MS 7500a, Agilent, USA). Rhodium was used as an internal standard for the indium measurement. The quantitative detection limit of indium was 0.045 µg/g for lung tissue. For all measurements, nitric acid blank and indium standards were prepared and concurrently tested with test samples. Tissues from the control group at different time points were dissolved similarly. The tissue concentration of indium was calculated using the following equations: [indium] treated tissue (μg/g wet tissue) = [indium] tissue suspension/wet weight of tissue.
Icp ms 7500a
Manufactured by Agilent Technologies
Sourced in United States
The Agilent ICP-MS 7500a is an inductively coupled plasma mass spectrometer designed for the analysis of trace elements in a variety of sample types. It offers high sensitivity and low detection limits for a wide range of elements.
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Lab products found in correlation
Icp oes, by Agilent Technologies (1 mentions)
Lc 1100 series, by Agilent Technologies (1 mentions)
Uv 3600 plus ultraviolet visible near infrared spectrophotometer, by Shimadzu (1 mentions)
Talos f200x, by Thermo Fisher Scientific (1 mentions)
Tecnai f20 microscope, by Thermo Fisher Scientific (1 mentions)
Empyrean diffractometer, by Malvern Panalytical (1 mentions)
Ht7700, by Hitachi (1 mentions)
Axis ultra dld, by Shimadzu (1 mentions)
Su8020 microscope, by Hitachi (1 mentions)
Asap 2010c, by Micromeritics (1 mentions)
Cary 60, by Agilent Technologies (1 mentions)
Jem 1200ex transmission electron microscope, by JEOL (1 mentions)
Originpro 9, by OriginLab (1 mentions)
10 protocols using icp ms 7500a
1
Quantifying Indium in Rat Organs
2
Microwave-Assisted Mineral Analysis of Seeds
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A detailed description of method used for mineral analysis is outlined as described by Carter et al. [19 (link)]. The ground seed samples were accurately weighed (0.3 g) into digestion Teflon vessels and concentrated nitric acid (4 mL) was added. The samples were digested using a microwave digestion system (MarsXpress, CEM, Matthews, NC, USA) programmed to three steps: step 1 (400 W power, 85 °C, 14 min), step 2 (800 W power, 110 °C, 20 min), and step 3 (1600 W power, 160 °C, 10 min) and the analysis was performed using inductively coupled plasma mass spectrometry (ICP-MS 7500a, Agilent, Tokyo, Japan) and optical emission spectrometry (ICP-OES, Varian Australia, VIC, Australia).
3
Arsenic Speciation in Algal Samples
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We determined total As and As speciation according to the previous methods used for algal sample preparation and As analysis [9 ]. In brief, approximately 0.02 g of oven-dried algal samples were treated overnight by means of microwave assisted digestion. Afterwards, samples were further diluted to measure As using ICP-MS. We had a good recovery rate (92.3% ± 5.6%) using a standard reference sample (GBW08521, the National Research Center for Standard Materials of China). Additionally, approximately 0.02 g of the freeze-dried algal samples was treated overnight. After microwave assisted digestion, samples were filtered using 0.45 μm filters. We then used HPLC-ICP-MS (Agilent LC1100 series coupled with the Agilent ICP-MS 7500a) to measure As speciation in algal extracts and media.
4
Bone Lipid and Mineral Analysis
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Bone marrow samples were homogenized at 4℃ (Bench top Homogenizer 300 DS PRO Scientific, Inc., Oxford, CT, USA). Total lipids from 0.8 g of the homogenized tissue were extracted [19 (link)]. Extracted lipids and oils blend samples were transmethylated, using 14% boron trifloride in methanol, to fatty acid methyl esters (FAMEs) [20 (link)]. FAMEs were separated by gas chromatography and fatty acid concentration was expressed as weight percentage of each fatty acid in total fatty acids.
Bone organ culture was performed as described in the literature [2 (link),9 (link)]. PGE2 analysis was performed in duplicate using a competitive enzyme-linked immunosorbent assay (ELISA) technique using a rabbit polyclonal antibody PGE2 kit (Oxford Biomedical Research, Inc., Oxford, MI, USA). PGE2 levels were expressed as ng/g bone.
Measurements of Ca, Mg, and Zn concentrations were performed in triplicate using an inductively coupled plasma mass spectrometer (ICP-MS 7500A, Agilent Technologies, Inc., Santa Clara, CA, USA), and P concentration was measured in triplicate using the molybdo-vanadate colorimetric method according to the AOAC [21 ]; optical density was measured at 410 nm using a UV/Visible 160A Shimadzu spectrophotometer, Kyoto, Japan. Mineral concentration was expressed as mg/g bone.
Bone organ culture was performed as described in the literature [2 (link),9 (link)]. PGE2 analysis was performed in duplicate using a competitive enzyme-linked immunosorbent assay (ELISA) technique using a rabbit polyclonal antibody PGE2 kit (Oxford Biomedical Research, Inc., Oxford, MI, USA). PGE2 levels were expressed as ng/g bone.
Measurements of Ca, Mg, and Zn concentrations were performed in triplicate using an inductively coupled plasma mass spectrometer (ICP-MS 7500A, Agilent Technologies, Inc., Santa Clara, CA, USA), and P concentration was measured in triplicate using the molybdo-vanadate colorimetric method according to the AOAC [21 ]; optical density was measured at 410 nm using a UV/Visible 160A Shimadzu spectrophotometer, Kyoto, Japan. Mineral concentration was expressed as mg/g bone.
5
Trace Metal Analysis of Tobacco
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The comminuted tobacco samples were digested with concentrated HNO3 and HClO4 (v/v, 4:1) [48 (link)]. Se and Cd contents were determined by inductively coupled plasma mass spectrometry (ICP-MS 7500A, Agilent, Palo Alto, CA, USA). The accuracy of elemental analysis was verified using standard reference materials from the China Standard Reference Center.
6
Advanced Spectroscopic Characterization of Electrocatalysts
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The extinction spectra were measured using a Shimadzu UV-3600 Plus ultraviolet/visible/near-infrared spectrophotometer with 0.5 cm optical path length plastic cuvettes. Transmission electron microscopy (TEM) imaging was carried out using a microscope (Hitachi HT 7700) operating at 120 kV. High-resolution transmission electron microscopy (HRTEM) imaging, high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) characterization and elemental mapping were performed using an FEI Tecnai F20 microscope operating at 200 kV, which was equipped with an Oxford energy-dispersive X-ray (EDX) analysis system. X-ray diffraction (XRD) was performed using a Panalytical Empyrean diffractometer. X-ray photoelectron spectroscopy (XPS) was performed using a SHIMADZU AXIS Ultra DLD. Selected area electron diffraction (SAED) patterns were captured using a ThermoFisher Talos F200X. The electrocatalysts were digested using aqua regia to dissolve the metals and then analyzed by the inductively coupled plasma optical emission spectrometer (ICP-OES) (Agilent ICP-MS 7500a).
7
Seawater Heavy Metal Analysis via ICP-MS
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The collected seawater samples were filtered through a 0.22 μm pore-size membrane filter, acidified by nitric acid before the measurement of heavy metals by ICP-MS (Agilent ICP-MS 7500a, America). A tuning solution was used to tune the ICP-MS to obtain the best analysis requirements for double charge, oxide, sensitivity, and resolution. The radio frequency power was 1, 350 W. The flow rates were 15.0 L•min - 1 for the plasma gas, 1.18 L•min -1 for the carrier gas, and 1.0 L•min -1 for the auxiliary gas. The sampling depth was 6.5 mm, the spray chamber temperature was 2.0 °C, the sample uptake rate was 1.00 mL•min -1 , the acquisition mode was the quantity, the integration time was 0.5 s, the dwell time was 30 ms, and the number of replicates was The luminous intensity of luminescent bacteria can be significantly affected by the salinity of water (Menz et al., 2013; Tan et al., 2016) . To keep consistence, salinity of the field collected seawaters was adjusted to 30 by adding solid sodium chloride to the samples to eliminate its effect on bio-luminescence of A. baylyi Tox2 (Table S8). The toxicity test method was performed as mentioned above.
8
Nanomaterial Dispersion in Hydrogels by LA-ICPMS
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The dispersion characteristic of inorganic nanoparticles in hydrogels was analyzed by LA-ICPMS. Briefly, 100 μL of the gel solution was transferred to a cover glass. The size and shape of the test samples were similar to those formed using a 48 well plate. Then, the slide was placed into the incubator to allow for gelation. A laser ablation system (Model: UP-213, New Wave Re- search Inc., Fremont, CA, Nd:YAG 213 nm wavelength) was used for sample ablation and the ablated fouling samples were introduced into a inductively coupled plasma mass spectrometer (7500a ICP-MS, Agilent Technology, Inc. USA) for element determination. The line scan mode was conducted under the following condition: a pulse rate of 10 Hz, a 150 μm diameter beam at a traveling velocity 100 μm/s, energy output 4 J/cm2, focused spot size 110 μm, transport Ar gas flow 1.01 L/min, dwell time 8 s, and intersite pause 1 s. The LA-ICP-MS scan range was 3000 μm × 2850 μm and a total of 900 lines covered the entire area of the tested gel sample. The raw data obtained from LA-ICP-MS were converted into a two-dimensional (2D) image by using the MATrix LABoratory (MATLAB) software.
9
Comprehensive Characterization of Adsorbent Materials
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The cations in real water and in the adsorption samples were examined by an Agilent 7500a ICP-MS instrument. Phosphate ions were detected with a UV–vis spectrophotometer (Agilent Cary 60) through the molybdenum blue method. The specific surface area was analyzed by a Brunauer-Eett-Teller (BET) method with a Micromeritics ASAP-2010C automatic analyzer (Micromeritics Col Inc., Australia). PXRD patterns of the samples were recorded in a MIXima XRD-7000 diffractometer between 10° and 70° (2θ) using CuKα radiation. FTIR spectra of samples were determined by a Nicolet Magna 550 FT-IR instrument in the KBr phase. SEM images and EDX of samples were examined with an SU8020 microscope and EDAX instruments, respectively (Hitachi, Japan). TEM images were determined using a JEM 1200EX transmission electron microscope (JEOL, Japan).
10
In situ Boron Mapping in Citrus Leaves
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For in situ B distribution, fresh main‐veins of boric acid‐treated Citrus leaves were transversely sectioned (24 µm) with an MEV freezing microtome (Slee Medical, Mainz, Germany). Cryosections were mounted on poly‐l ‐lysine‐coated glass slides and then freeze‐dried at −40°C immediately to minimize B diffusion. Prepared slides were photographed with a light microscope before being applied to GeoLasPro LA‐ICP‐MS (Coherent, Santa Clara, CA, USA). Laser ablation was performed in line scanning mode with carrier helium gas flow of 600 ml min−1, laser power set to 20% and laser spot diameter of 20 µm. The 7500a ICP‐MS (Agilent) was set up in time‐resolved analysis mode, and the resulting amounts of B were reported in counts per second. Finally, a B distribution contour was generated from the ICP‐MS data that represents the total count of the B signal at each time point using Origin Pro 9.0 (Origin Lab, Northampton, MA, USA).
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