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Substance p acetate salt hydrate

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Substance P acetate salt hydrate is a chemical compound used in laboratory research. It is a salt form of the neuropeptide Substance P, which plays a role in various physiological processes. The product is available in a hydrated state.

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Lab products found in correlation

Capsaicin, by Merck Group (2 mentions) Resiniferatoxin, by Merck Group (1 mentions) Win 62 577, by Merck Group (1 mentions) Human angiotensin 2, by Merck Group (1 mentions) Resiniferatoxin rtx, by Merck Group (1 mentions) Evans blue dye, by Merck Group (1 mentions) Cp99994, by Bio-Techne (1 mentions) Neurotensin, by Merck Group (1 mentions) Angiotensin 1 human acetate salt hydrate, by Merck Group (1 mentions) Melittin, by Merck Group (1 mentions) Acetylcholine chloride, by Merck Group (1 mentions) Nifedipine, by Merck Group (1 mentions) Papaverine hydrochloride, by Merck Group (1 mentions) Sodium nitroprusside dehydrate, by Merck Group (1 mentions) U46619, by Bio-Techne (1 mentions) Nω nitro l arginine methyl ester hydrochloride l name, by Merck Group (1 mentions) Phenylephrine hydrochloride, by Merck Group (1 mentions) Arg8 vasopressin acetate salt, by Merck Group (1 mentions) L norepinephrine hydrochloride, by Merck Group (1 mentions) Indomethacin, by Merck Group (1 mentions)

Close spelling variants of this product

Acetate salt hydrate Substance P

7 protocols using substance p acetate salt hydrate

1

Substance P Application in ACSF

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Substance P acetate salt hydrate (Sigma) was dissolved in ACSF and applied by exchanging solutions via a three-way stopcock using a modified chamber adapted for pharmacological experiments that limits drug dilution [22 (link)].
Hachisuka J., Koerber H.R, & Ross S.E. (2020). Selective-cold output via a distinct subset of lamina I spinoparabrachial neurons. Pain, 161(1), 185-194.
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2

Evaluation of L. reuteri compound effects

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L. reuteri DSM 17938 (Biogaia AB, Stockholm, Sweden) was purchased from Aché Pharmaceutical Laboratories (São Paulo, Brazil). Ethanol, Substance P acetate salt hydrate, Resiniferatoxin (RTX), and non-peptide NK1 tachykinin receptor antagonist (WIN 62,577) were purchased from Sigma Aldrich (St. Louis, MO, USA). The other reagents were of analytical grade bought from standard commercial companies. One milligram of RTX was dissolved in 1 mL of 95% ethanol and stored at −20 °C. When needed, this solution was diluted in 0.9% saline to the necessary concentration. WIN 62,577 was dissolved in dimethyl sulfoxide (DMSO). The other compounds were dissolved in saline when needed.
Oliveira A.P., Souza L.K., Araújo T.S., de Araújo S., Nogueira K.M., Sousa F.B., Silva R.O., Pacífico D.M., Martins C.S., Brito G.A., Souza M.H, & Medeiros J.V. (2019). Lactobacillus reuteri DSM 17938 Protects against Gastric Damage Induced by Ethanol Administration in Mice: Role of TRPV1/Substance P Axis. Nutrients, 11(1), 208.
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3

Quantitation of Molecular Compounds

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D8-ciprofloxacin hydrochloride
hydrate, angiotensin II (human), substance P acetate salt hydrate,
CHCA, and 3,5-diiodo-l-tyrosine dihydrate were purchased
from Sigma-Aldrich. Ciprofloxacin and enrofloxacin were obtained from
Energy Chemical (Shanghai, China). Sarafloxacin was purchased from
J&K Scientific Co. Ltd. (Beijing, China). β-cyclodextrin
was purchased from Aladdin ( Shanghai, China). C60 was
purchased from TCL (Shanghai, China).
Yang S., Mu L., Feng R, & Kong X. (2019). Selection of Internal Standards for Quantitative Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Analysis Based on Correlation Coefficients. ACS Omega, 4(5), 8249-8254.
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4

Dyes, Peptides, and Antagonists in Neurological Studies

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Evans blue dye (50 mg/ml saline; Sigma-Aldrich, St. Louis, MO, United States), human calcitonin gene-related peptide (CGRP; 1 mg/ml saline; Bachem, Torrance, CA, United States; a 37-amino acid peptide), and α-CGRP 8-37 (1 mg/ml distilled water, Bachem, Torrance, CA, United States; a CGRP receptor antagonist) were used in this study (Shen et al., 2001 (link)). Substance P acetate salt hydrate (SP; 0.5 mg/ml saline; Sigma-Aldrich; a SP receptor agonist) and (+)-(2S,3S)-3-(2-methoxybenzylamino)-2-phenylpiperidine (CP-99994; 33 mmol/ml saline; Sigma-Aldrich; an SP receptor antagonist) were also used (McLean et al., 1993 (link)). Resiniferatoxin (RTX; 100 μg/ml vehicle; Sigma-Aldrich), an ultrapotent Capsaicin analog known to block small diameter afferent fibers containing transient receptor potential vanilloid type 1 receptor (TRPV1) (Suter et al., 2009 (link)), was dissolved in a vehicle that contained 0.3% Tween 80, 10% DMSO and saline. Capsaicin (0.05 and 0.1%; Sigma-Aldrich; a TRPV1 agonist) was dissolved in a vehicle consisting of 10% alcohol and 10% Tween 80 in saline (Knotkova et al., 2008 (link)), and FITC-conjugated isolectin B4 (FITC IB4 tracer; 1%, 3 μl, Vector Laboratories, Burlingame, CA, United States) was used.
Fan Y., Kim D.H., Ryu Y., Chang S., Lee B.H., Yang C.H, & Kim H.Y. (2018). Neuropeptides SP and CGRP Underlie the Electrical Properties of Acupoints. Frontiers in Neuroscience, 12, 907.
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5

Substance P Modulation of Pain Responses

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Evans blue dye (EBD; 50 mg/ml in saline; Sigma-Aldrich, USA), the SP receptor agonist substance P acetate salt hydrate (SP; 0.5 mg/ml in saline; 30 μL/loci, subcutaneous (s.c.); Sigma-Aldrich), the SP receptor antagonist (+)-(2S, 3S)-3-(2-methoxybenzylamino)-2-phenylpiperidine (CP-99994; 33 mmol/ml in saline; 30 μL/loci, s.c.; TOCRIS, UK), were used (McLean et al., 1993 ). The transient receptor potential channel and vanilloid subfamily member 1 (TRPV1) agonist capsaicin (0.05%; 20 μL/loci, s.c.; Sigma-Aldrich), was dissolved in vehicle consisting of 10% alcohol and 10% Tween 80 in saline.
Fan Y., Kim D.H., Seob Gwak Y., Ahn D., Ryu Y., Chang S., Lee B.H., Bills K.B., Steffensen S.C., Yang C.H, & Kim H.Y. (2020). The role of substance P in acupuncture signal transduction and effects. Brain, behavior, and immunity, 91, 683-694.
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6

Peptide Solutions for Mass Spectrometry

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Angiotensin I human acetate salt hydrate, [Met5]enkephalin acetate
salt hydrate, neurotensin, substance P acetate salt hydrate, and melittin from honey bee
venom were purchased from Sigma-Aldrich (St. Louis, MO, USA). Solutions of angiotensin I
and methionine enkephalin were used in concentrations of 1 µM. A four peptide
mixture solution was made from 1 µM each angiotensin I, neurotensin, substance P,
and melittin. Each solution was made in a matrix of 50:50:1 methanol:water:acetic acid and
samples were used without further purification.
Webb I.K., Chen T.C., Danielson WF I.I.I., Ibrahim Y.M., Tang K., Anderson G.A, & Smith R.D. (2014). Implementation of Dipolar Resonant Excitation for Collision Induced Dissociation with Ion Mobility/Time-of-Flight MS. Journal of the American Society for Mass Spectrometry, 25(4), 563-571.
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7

Wire Myography Buffer Compositions

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Two types of buffers were used for wire myography experiments, physiological salt solution (PSS) and high potassium physiological salt solution (KPSS). The composition of PSS (in mM) is sodium chloride 119, potassium chloride 4.7, magnesium sulfate heptahydrate 1.17, sodium bicarabonate 25, potassium dihydrogen orthophosphate 1.18, EDTA 0.027, D-(+)-glucose 5.5, calcium chloride dehydrate 2.5. For KPSS, sodium chloride was replaced with 123.7 mM potassium chloride. Both were prepared according to protocols developed by Mulvany [3] . (R)-(-)-Phenylephrine hydrochloride (P6126), [Arg 8 ]-Vasopressin acetate salt (V9879), acetylcholine chloride (A6625), angiotensin II human (A9525), bradykinin acetate salt (B3259), histamine (H7125), indomethacin (I7378), L-norepinephrine hydrochloride (74480), nifedipine (N7634), NΩ-nitro-L-arginine methyl ester hydrochloride (L-NAME) (N5751), papaverine hydrochloride (P3510), riluzole (R116), sodium nitroprusside dehydrate (S0501) and Substance P acetate salt hydrate (S6883) were bought from Sigma, UK. U46619 (1932) was bought from Tocris, UK. Endothelin-1 (human, porcine) (ab120471) was purchased from Abcam, UK. Serotonin (hydrochloride) (14332) was bought from Cayman, US. SQ29548 (BML-RA103) was purchased from Enzo, UK.
Sander K.N., Ali T.Y., Warren A.Y., Hay D.P., Broughton Pipkin F., Barrett D.A, & Khan R.N. (2019). Pharmacological profile of vascular activity of human stem villous arteries. Placenta, 88.
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