Rabbit anti atg7

Manufactured by Abcam

Sourced in United Kingdom, United States

Rabbit anti-ATG7 is a primary antibody that targets the ATG7 protein. ATG7 is an E1-like enzyme that is essential for the autophagy process. This antibody can be used to detect and study the expression of ATG7 in various experimental systems.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Anti-ATG7 (15 citations) Rabbit anti- (5 citations)

3 protocols using rabbit anti atg7

Western Blot Protein Expression Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blot analysis was performed to determine the protein expression as previously described (33 (link)). The total proteins were extracted with radioimmunoprecipitation assay (RIPA) lysis buffer containing protease and phosphatase inhibitor cocktail (Beyotime), and quantified using BCA Kit (Beyotime), followed by separation on SDS-PAGE and transferring to polyvinylidene difluoride membranes (Millipore, Billerica, MA, USA), which were then reacted with different primary antibodies at 4 °C overnight. The primary antibodies included mouse anti-myosine heavy chain (MHC) antibody (R&D Systems, Minneapolis, MN, USA), rabbit anti-MuRF1, rabbit anti-MAFbx (Fbx32), rabbit anti-LC3B, rabbit anti-PINK1, rabbit anti-BNIP3, rabbit anti-ATG7, rabbit anti-Beclin 1, rabbit anti-beta tubulin (Abcam, Cambridge, UK), mouse anti-pJak1 (Tyr1034/1035)/Jak2 (Tyr1007/1008), rabbit anti-pSTAT3 (Tyr705), and rabbit anti-Stat3 (Cell Signaling Technology, Danvers, MA, USA). Then, the membranes were incubated with horse radish peroxidase (HRP)-conjugated secondary immunoglobin G (IgG) antibodies for 2 h. The target proteins were detected using enhanced chemiluminescence (Thermo Fisher Scientific, USA), and the band intensity was quantified and normalized against loading control.

Huang Z., Zhong L., Zhu J., Xu H., Ma W., Zhang L., Shen Y., Law B.Y., Ding F., Gu X, & Sun H. (2020). Inhibition of IL-6/JAK/STAT3 pathway rescues denervation-induced skeletal muscle atrophy. Annals of Translational Medicine, 8(24), 1681.

+ Open protocol

+ Expand

Immunofluorescence Analysis of Autophagy Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies were as follows: mouse anti-a2V (Covance, Denver, PA); rabbit anti-GAPDH (Cell signaling, Danvers, MA); rat anti-F4/80, rabbit anti-LC3B, rabbit anti-LAMP-1, rat anti-LAMP-2, rabbit anti-ATG3, rabbit anti-ATG4, rabbit anti-ATG7, rabbit anti-NF-κB p65 (all from Abcam). Secondary antibodies were as follows: goat anti-rabbit IgG-FITC, -mouse IgG AF-594, -rabbit IgG AF-594 (Invitrogen), rabbit anti-rat IgG-FITC (Abcam), goat anti-rabbit, -rat, -mouse IgG-HRP (Santa Cruz Biotechnology), donkey anti-rabbit IRDye-800CW (LI-COR Bioscience, Lincoln, NE) and EnVision + dual link System-Horseradish peroxidase (HRP) (Dako). Isotype control antibodies were as follows: control mouse IgG (R&D Systems); rat IgG isotype, mouse IgG isotype and rabbit IgG isotype (Abcam).

Agrawal V., Jaiswal M.K., Mallers T., Katara G.K., Gilman-Sachs A., Beaman K.D, & Hirsch E. (2015). Altered autophagic flux enhances inflammatory responses during inflammation-induced preterm labor. Scientific Reports, 5, 9410.

+ Open protocol

+ Expand

Protein Extraction and Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The total protein in the muscle was extracted from the protein lysate. Protein concentration was measured using a BCA kit (Beyotime, Haimen, China). Thereafter, 30 μg of the total protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene difluoride (PVDF) membrane via the wet transfer method. The PVDF membrane was blocked for 1 h, followed by incubation with the primary antibody antibodies: mouse anti-MyHC (R&D Systems, Minneapolis, MN), rabbit anti-NOX4 (Invitrogen, Rockford, IL, USA), rabbit anti-MAFbx, rabbit anti-PINK1, rabbit anti-ATG7, mouse anti-BNIP3, rabbit anti-LC3B, rabbit anti-NOX2, rabbit anti-Nrf2, rabbit anti-NQO1, rabbit anti-PGC-1α (Abcam, Cambridge, UK), mouse anti-p-Jak1 (Tyr1034/1035)/Jak2 (Tyr1007/1008), rabbit anti-p-Stat3 (Tyr705) and rabbit anti-Stat3 (Cell Signaling technology, MA, USA), rabbit anti-MuRF-1, mouse anti-Troponin I-FS, mouse anti-Troponin I-SS, and mouse anti-tubulin (Santa Cruz, Santa Cruz, CA) at 4°C overnight. On the following day, the PVDF membrane was rinsed thrice with tris-buffered saline with Tween (TBST) and incubated with the corresponding secondary antibody at room temperature for 1 h. Thereafter, PVDF membrane was rinsed thrice with TBST, treated with the appropriate amount of luminescent liquid, and finally scanned using a membrane scanner.

Shen Y., Zhang Q., Huang Z., Zhu J., Qiu J., Ma W., Yang X., Ding F, & Sun H. (2020). Isoquercitrin Delays Denervated Soleus Muscle Atrophy by Inhibiting Oxidative Stress and Inflammation. Frontiers in Physiology, 11, 988.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!