Anti mip 1β percp cy5

Manufactured by BD

Anti-MIP-1β-PerCP-Cy5.5 is a fluorescently-labeled antibody that binds to the Macrophage Inflammatory Protein-1 beta (MIP-1β) protein. The PerCP-Cy5.5 fluorescent dye is conjugated to the antibody, allowing for the detection and quantification of MIP-1β-expressing cells using flow cytometry.

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Lab products found in correlation

Anti cd3 bv605 clone okt3, by BioLegend (1 mentions) Anti cd56 pe cy7 clone ncam16, by BD (1 mentions) Anti cd3 buv395 clone ucht1, by BD (1 mentions) Anti ifn γ v450, by BD (1 mentions) Anti ifn γ apc, by BioLegend (1 mentions) Intracellular staining permeabilization wash buffer, by BioLegend (1 mentions) Cd28h antibody, by R&D Systems (1 mentions) Anti cd56 bv421, by BD (1 mentions) Lsrfortessa x 20, by BD (1 mentions) Golgistop, by BD (1 mentions) Anti cd107a pe, by BD (1 mentions)

Close spelling variants of this product

MIP-1β PerCP-Cy5.5

2 protocols using anti mip 1β percp cy5

Comprehensive Immune Profiling using Multicolor Flow Cytometry

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The following anti-human antibodies were used in this study: anti-CD3-BV605 (clone OKT3, Biolegend), anti-CD3-BUV395 (clone UCHT1, BD Bioscience), anti-CD14-V500 (clone M5E2, BD Bioscience), anti-CD19-BV510 (clone SJ25C1, BD Bioscience), anti-CD56-PE-Cy7 (clone NCAM16.2, BD Bioscience), anti-CXCR6-PE or anti-CXCR6-APC (clone K041E5, Biolegend), anti-CD57-AF488 (clone TB01, eBioscience), anti-TRAIL-BV421 (clone RIK-2, BD Bioscience), anti-CD49a-FITC (clone TS2/7, Biolegend), anti-HLA-DR V500 (clone G46-6, BD Bioscience) and anti-CD69-PE-Dazzle (clone FN50, Biolegend) for surface antigens; and anti-T-bet-eFlour610 (clone 4B10, eBioscience) and anti-Eomes-PE-eFlour610 (clone Dan11mag, eBioscience) for intranuclear antigens; anti-IFNγ-V450 (BD Bioscience), anti-MIP-1β-PerCP-Cy5.5 (cloneD21–1351, BD Bioscience), anti-GM-CSF-PE-CF594 (clone BVD2–21C11, BD Bioscience), anti-TNF-FITC (clone MAb11, BD Bioscience), anti-Granzyme B-AlexaFlour700 (clone GB11, BD Bioscience) and anti-Perforin-PerCP-Cy5.5 (clone dG9, Biolegend) for intracellular antigens.

Stegmann K.A., Robertson F., Hansi N., Gill U., Pallant C., Christophides T., Pallett L.J., Peppa D., Dunn C., Fusai G., Male V., Davidson B.R., Kennedy P, & Maini M.K. (2016). CXCR6 marks a novel subset of T-betloEomeshi natural killer cells residing in human liver. Scientific Reports, 6, 26157.

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Measuring NK Cell Activation and Function

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Resting NK cells were mixed with transfected S2 cells at an E:T ratio of 1:2. Cells were incubated in IMDM medium with 10% FCS for 2 hours, stained with Live/Dead-NIR, anti-CD56-Bv421 and anti-CD107a-PE, and analyzed by flow cytometry. To stain for intracellular cytokines and chemokines, NK cells and S2 cells were incubated for 1 hour, and in the presence of 3 μM GolgiStop (BD Biosciences) for 5 hours. For CD28H blocking, 10 μg/ml CD28H antibody (R&D Systems) was pre-incubated with NK cells for 15 min before mixing with S2 cells. Cells were stained with anti-CD107a-PE (BD 555801) and anti-CD56-Bv421 (BD 562751), fixed with 4% paraformaldehyde for 10 min, and permeabilized with the Intracellular Staining Permeabilization Wash Buffer (BioLegend). Cells were stained with anti-IFN-γ-APC (BioLegend 506510), anti-TNF-⍺-BV650 (BioLegend 502937), anti-MIP-1α-FITC (Invitrogen MA523564) and anti-MIP-1β-PerCP-Cy5.5 (BD 560688). Data were obtained on a LSR II (BD Biosciences) or LSRFortessa™ X-20 (BD Biosciences), and analyzed with FlowJo (FlowJo, LLC).

Zhuang X, & Long E.O. (2019). CD28 homolog is a strong activator of natural killer cells for lysis of B7H7+ tumor cells. Cancer immunology research, 7(6), 939-951.

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