Pla polymer

Lyso-7, [(5Z)-5-[5-bromo-1H-indol-3-yl)methylene]-3-(4-chlorobenzyl)-thiazolidine-2,4-dione] (CAS Number 1505484-42-3) (CAS Registry Number 1505484-42-3), Mw 447.73 g/mol, was synthesized, purified and characterized by Prof Ivan da Rocha Pitta as reported [11 (link)]. PLA polymer, a poly-rac-lactide Mn 75,000–120,000 g/mol, poloxamer 188, polysorbate 80, polyethylene glycol 300 (PEG 300), glucose, ethanol and methanol (HPLC grade) were purchased from Sigma-Aldrich (Cotia, Brazil). Symplicity^® System (Millipore, Bedford, MA, USA) produced Milli-Q water to prepare all solutions throughout the experiments. Soy lecithin with 75% of phosphatidylcholine (Epikuron^® 170) was a generous gift from Lucas Meyer (Champlan, France). Sasol Olefins & Surfactants GmbH (Hamburg, Germany) provided Miglyol 810 N. Tedia (Rio de Janeiro, Brazil) provided ethyl acetate, acetone, N,N-dimethylacetamide (DMA), acetone and dimethylsulfoxide (DMSO) (analytical grade).

PLA polymer (Sigma–Aldrich) was dissolved 10% w/v in dichloromethane (DCM). Fifty microlitre of Span80 (Sigma–Aldrich) was added to the polymer solution and stirred at 250 rpm for 10 min. l-ascorbic acid (Sigma–Aldrich) and l-ascorbic acid 2-phosphate (SigmalAldrich) were dissolved in distiled water and a total volume of 500 μl solution was added drop wise to the PLA-Span80 solution while stirring 1000 rpm with magnets for 15 min (Fig. 1), the final concentration being 0.0001, 0.001 and 0.01 g of either AA or A2P per gram of PLA. Unless stated otherwise, the medium concentration (0.001 g of AA and A2P per gram of PLA) was used in experiments. A control emulsion electrospun scaffold containing only 500 μl dH₂O (Vehicle scaffolds), without AA or A2P was also included together with a PLA only electrospun scaffold. All emulsions were freshly made and electro spun immediately.