P800 automated biochemistry analyzer

A structured questionnaire was used to obtain participants’ demographic information and clinical characteristics. A smoker was defined as a person who smoked at least one cigarette per day in the past 1 month, or a past smoker even if the participant had completely abstained from cigarette use for at least 1 month. An alcoholic drinker was defined as a person who consumed any kind of alcoholic beverage on average more than once a week. We obtained samples of fasting blood from all participants. MODULE P800 automated biochemistry analyzer (ROCHE, USA) was used to measure plasma lipid concentrations of all participants. Total cholesterol (TC) and serum triglycerides (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) profiles from both cases and controls were measured.

Fasting blood samples of each respondent were drawn by venipuncture to measure serum Total Cholesterol (TC), High Density Lipoprotein Cholesterol (HDL-C), Low Density Lipoprotein Cholesterol (LDL-C) and Triglycerides (TG). The tools used for all blood samples collection were from the same source. After collection, the samples were placed in a cold chain system before being collectively transported to a central laboratory. Blood samples were analyzed using MODULE P800 automated biochemistry analyzer (ROCHE, USA).