Sodium alginate salt

Manufactured by Merck Group

Sourced in Germany

Sodium alginate salt is a naturally derived, water-soluble polysaccharide. It is extracted from brown seaweed and functions as a thickening, stabilizing, and emulsifying agent in various applications.

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Sodium alginate (718 citations) Alginate (156 citations) Alginate acid sodium salt (3 citations) Alginic acid sodium salt (alginate; (2 citations)

6 protocols using sodium alginate salt

Determination of Ascorbic Acid Content

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The L-ascorbic acid (AA) (100% purity), sodium alginate salt from brown algae (ALG) and gum arabic (GA) were supplied by Sigma-Aldrich (Budapest, Hungary). The 2-6-dichlorophenol-indophenol sodium salt glacial acetic acid, metaphosphoric acid and sodium hydrogen carbonate were supplied by Merck Millipore, Darmastardt, Germany. All reagents were of analytical grade and used without any further purification.

Barra P.A., Márquez K., Gil-Castell O., Mujica J., Ribes-Greus A, & Faccini M. (2019). Spray-Drying Performance and Thermal Stability of L-ascorbic Acid Microencapsulated with Sodium Alginate and Gum Arabic. Molecules, 24(16), 2872.

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Chitosan Sulfate Synthesis and Characterization

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Food-grade chitosan with a 90% degree of deacetylation was purchased from América Alimentos (Zapopan, Mexico). Acetic acid (Fermont, Monterrey, Mexico), dimethylformamide (Fluka, Buchs, Germany), chlorosulfonic acid (Sigma-Aldrich, St. Louis, MO, USA), and methanol (Fermont, Monterrey, Mexico) were used as received. Chitosan sulfate (Figure 1) was synthesized from chitosan using the procedure previously reported [17 ]. Sodium alginate salt was obtained from Sigma Aldrich (Irvine, United Kingdom). Calcium chloride was obtained from Fermont (Monterrey, Mexico). Copper(II) ion solutions were prepared with copper sulfate pentahydrate salt (Fermont, Monterrey, Mexico) and bi-distilled water. The pH was adjusted with a 0.1 M HCl solution.

Verduzco-Navarro I.P., Rios-Donato N., Jasso-Gastinel C.F., Martínez-Gómez Á.D, & Mendizábal E. (2020). Removal of Cu(II) by Fixed-Bed Columns Using Alg-Ch and Alg-ChS Hydrogel Beads: Effect of Operating Conditions on the Mass Transfer Zone. Polymers, 12(10), 2345.

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Preparation of Sodium Alginate BioInk

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Sodium alginate salt (2000 cP viscosity, was bought from the (Sigma-Aldrich, USA). The BioInk solution was produced from grade type III ASTM water i.e. (Wasserlab, Spain) . The alginate solution was mixed by mechanical stirring overnight under (Scilogex, USA, 200 rpm) at temperature of (25 °C).

Datta S., Jana S., Das A., Chakraborty A., Chowdhury A.R, & Datta P. (2020). Bioprinting of radiopaque constructs for tissue engineering and understanding degradation behavior by use of Micro-CT. Bioactive Materials, 5(3), 569-576.

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Bioconjugation of Polymers for Immunoassay

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All chemicals are used without further purification, unless stated otherwise. Alginate sodium salt, formaldehyde (37%), hydrochloric acid (HCl), dimethyl sulfoxide (DMSO), 3,3’,5,5’-tetramethylbenzidine (TMB), and hydrogen peroxide (30% w/w) were purchased from Sigma Aldrich/Merck, St. Louis, MO, United States. Hyaluronic acid sodium salt (40–65 kDa) was purchased from LifeCore, Kloten, Switzerland. Chitosan (90/30/A1) was purchased from BioLog Heppe, Landsberg, Germany. Poly(ethylene glycol) diamine with molecular weights of 2000, 1000, and 600 g mol⁻¹, as well as poly(ethylene glycol) dialdehyde with a molecular weight of 1000 g mol⁻¹, and carboxylic acid poly(ethylene glycol) biotin with a molecular weight of 1000 g mol⁻¹ were purchased from Creative PEGWorks, Chapel Hill, NC, United States. Tert-butyl isocyanide was purchased from TCI Deutschland, Eschborn, Germany. Streptavidin Alexa Fluor 555 conjugate was purchased from Invitrogen, Carlsbad, CA United States. Streptavidin-HRP DY998 was purchased from R&D Systems, Minneapolis, MN, United States. Deionized water from a Milli-Q water purifier with a resistance of 18.2 MΩ cm was used.

Hauck N., Seixas N., Centeno S.P., Schlüßler R., Cojoc G., Müller P., Guck J., Wöll D., Wessjohann L.A, & Thiele J. (2018). Droplet-Assisted Microfluidic Fabrication and Characterization of Multifunctional Polysaccharide Microgels Formed by Multicomponent Reactions. Polymers, 10(10), 1055.

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Synthesis and Characterization of Peptide-Lipid-starPEG Constructs

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All chemicals were used without further purification unless stated otherwise. 4-Arm poly(ethylene glycol)-thiol (starPEG-thiol) with a molecular weight of 10 000 g mol⁻¹ and 4-arm poly(ethylene glycol)-maleimide (starPEG-maleimide) with 10 000 g mol⁻¹ were purchased from Creative PEGWorks (USA) and Jenkem (China). Alexa Fluor 488 C5 maleimide was purchased from Thermo Fisher Scientific (USA). Alginate sodium salt, Brilliant Black, calcium chloride, phosphate-buffered saline (PBS) tablets, dextran sulfate sodium salt with a molecular weight of 5000 g mol⁻¹, and fluorescein isothiocyanate-(FITC-)dextran with an average molecular weight of 2 000 000 g mol⁻¹ were purchased from Sigma Aldrich/Merck (Germany). Peptide–lipid-starPEGs were synthesized in the Zhang lab (B CUBE, Technische Universität Dresden). Deionized (DI) water was obtained from a Milli-Q water purifier (Direct-Q® 5, Merck Millipore, USA) with a resistance of 18.2 MΩ cm.

Hauck N., Neuendorf T.A., Männel M.J., Vogel L., Liu P., Stündel E., Zhang Y, & Thiele J. (2021). Processing of fast-gelling hydrogel precursors in microfluidics by electrocoalescence of reactive species. Soft Matter, 17(45), 10312-10321.

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Biopolymer-Based Drug Delivery System

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Alginate sodium salt (medium viscosity), O- carboxymethyl chitosan (DS = 34%), diclofenac sodium (DS; assay 99%), calcium chloride (anhydrous; assay 98%) were acquired from Sigma-Aldrich (Taufkirchen, Germany). Aminated chitosan (AmCs; low viscosity) was provided by ATNMRI, SRTA-City (Cairo, Egypt). Acetic acid (assay 98%), hydrochloric acid (assay 37%), di-sodium hydrogen phosphate (anhydrous; ≥99%), sodium di-hydrogen phosphate (≥99%), citric acid (assay 99%), hydrochloric acid, and potassium chloride (assay 99%) were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China).

Omer A.M., Ahmed M.S., El-Subruiti G.M., Khalifa R.E, & Eltaweil A.S. (2021). pH-Sensitive Alginate/Carboxymethyl Chitosan/Aminated Chitosan Microcapsules for Efficient Encapsulation and Delivery of Diclofenac Sodium. Pharmaceutics, 13(3), 338.

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