Bovine anti rabbit igg fitc

IF stain was used to determine the expression of CD29, CD44, and CD45 in mouse prostate tissue. Mouse prostate paraffin sections (8 μm) were fixed in acetone for 10 min and rinsed 3 times with PBS. Then, the tissue sections were, respectively, incubated at 4°C overnight with rabbit anti-mouse CD29, CD44, and CD45 monoclonal antibodies (1 : 100, Bioss Biotechnology Ltd., Beijing, China). Sections were rinsed with PBS and incubated with bovine anti-rabbit IgG-FITC (Santa Cruz, USA) for 30 min at room temperature. Finally, sections were incubated with DAPI and observed with a fluorescent microscope.

Goat anti-rat S100A8, mouse anti-rat granulocyte (HIS48)-FITC, mouse anti-rat macrophage subset (HIS36)-FITC (ED2 antigen), goat anti-rat CD68, rabbit anti-rat CD14, bovine anti-goat IgG-TR, bovine anti-goat IgG-PE, bovine anti-goat IgG-FITC, bovine anti-rabbit IgG-FITC, and normal IgG were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

After 48 h post-radiation, cultured cells were washed twice with phosphate-buffered saline (PBS), fixed in 4% paraformaldehyde for 15 min at room temperature, and then permeabilized by incubation for 15 min with 0.01% Tween 20 in PBS. Samples were blocked with 5% bovine serum albumin followed by incubation with anti-collagen I, anti-phospho-Smad 2/3 and anti-β-catenin (1:100, Abcam, Cambridge, MA, USA) overnight at 4 °C. The next day, cells were washed with PBS and incubated with bovine anti-rabbit IgG-FITC (1:200, Santa Cruz Biotechnology) secondary antibody for 2 h at room temperature. Cells were mounted on slides with mounting solution containing DAPI (Vector Laboratories, Burlingame, CA, USA), and cells were viewed under a confocal microscope system (LSM700, Olympus, Center Valley, PA, USA).