The human ER+ breast cancer cell lines MCF-7, T47D, ZR75–1, and BT474, were obtained from Dr. Debra Tonetti (University of Illinois at Chicago). HCC1428 cells were purchased from ATCC. These cells were routinely maintained in RPMI 1640 media (Invitrogen Life Technologies) with phenol red supplemented with 10% FBS, 1% non-essential amino acids, 2 mmol/L L-glutamine, 1% antibiotics penicillin-streptomycin, and 6 ng/mL insulin. NFκB-RE-GFP cells were obtained from Dr. Elaine T. Alarid (University of Wisconsin-Madison). These cells were generated from MCF-7 cells stably transfected with 3X-κB reporter, which has three enhancer elements (Igκ, IκBα and the palindromic consensus sequence) upstream of the thymidine kinase promoter driving green fluorescent protein (GFP) expression, as described in (16 (link)). All cell lines are routinely authenticated by short tandem repeat analysis and tested for mycoplasma using LookOut Mycoplasma PCR Detection Kit (Sigma).
Hcc1428 cells
Manufactured by American Type Culture Collection
HCC1428 cells are a human breast cancer cell line derived from a 55-year-old Caucasian female patient. They are epithelial-like in morphology and exhibit anchorage-dependent growth. The cells express the estrogen receptor and progesterone receptor.
Automatically generated - may contain errors
2 protocols using hcc1428 cells
1
Characterization of ER+ Breast Cancer Cell Lines
2
Engineered Cell Lines for Estrogen Receptor Mutant Studies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Elacestrant (RAD1901) ((6R)-6-(2-(N-(4-(2-(ethylamino)ethyl)benzyl)-N-ethylamino)-4-methoxyphenyl)5,6,7,8-tetrahydronaphthalen-2-ol dihydrochloride) was manufactured by Patheon. Elacestrant lots used in this study were periodically checked to ensure purity, stability, and chirality. HCC1428 cells were purchased from ATCC, and HCC1428-LTED (long-term estrogen deprived) were developed by maintaining the cells in RPMI phenol red-free medium supplemented with 10% charcoal-stripped FBS (HyClone, GE Healthcare Life Sciences) and 1% pen-strep (Thermo Fisher Scientific) at 5% CO2. MCF7 cells harboring wild-type ER were genetically modified using CRISPR-Cas9 to express either the ESR1:Y537S or the ESR1:D538G mutated proteins. Briefly, single-guide RNAs were utilized to create the KI/KI cell line containing ESR1:Y537S and the KI/KO cell line containing ESR1:D538G, and single-cell clones were isolated and grown to establish these cells. The MCF7-Y537S/D538G cell lines were maintained in RPMI phenol red-free medium supplemented with 10% charcoal-stripped FBS (HyClone, GE Healthcare Life Sciences) and 1% pen-strep (Thermo Fisher Scientific) at 5% CO2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!