Cal33 and HSC-6 cells were transfected with 5 uM non-targeting small interfering RNA (siRNA) (OriGene-SR30004) or MAPK1-targeting siRNA (OriGene-SR303751) using the siTran1.0 siRNA Transfection Reagent (OriGene-TT300001) according to the manufacturer's protocol. Briefly, cells were apportioned into T25 flasks and transfected 24 hours later with 5 uM control siRNA or MAPK1-targeting siRNA. At 48 hours after transfection, cells were trypsinized and seeded into 48-well plates. ELISA quantification was performed using 24-hour conditioned media. In parallel, cells protein samples were isolated, and the efficiency of knockdown was analyzed by western blotting.
Sitran 1.0 sirna transfection reagent
Manufactured by OriGene
SiTran 1.0 is a siRNA transfection reagent. It facilitates the delivery of small interfering RNA (siRNA) into cells to modulate gene expression.
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Lab products found in correlation
3 protocols using sitran 1.0 sirna transfection reagent
1
MAPK1 Knockdown in Cell Lines
2
Transfecting ImSPEM cells with siRNA
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ImSPEM cells were plated on collagen-coated, 6-well plates the day before transfection to provide confluency of 60%–70% in 24 hours. ImSPEM cells were transfected with Slc7a11 Mouse siRNA Oligo Duplexes or Trilencer-27 Universal Scrambled Negative Control siRNA Duplex (SR416143; Origene, Rockville, MD) according to the manufacturer’s recommendations using siTran 1.0 siRNA transfection reagent (TT300001; Origene).
3
EGFR Knockdown in Cell Lines
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Cells were transfected with siTran 1.0 siRNA transfection reagent (Origene, Cat. No. TT300002) applying a siRNA directed against EGFR (Origene) according to the manufacturer’s instructions. For details see Supplementary Information.
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