Modified Boyden chamber assays were conducted using 24-well Transwell polyester membrane filter inserts with 8 µm pores and 0.33 cm2 surface area (Corning Inc., Corning, NY, USA) at a density of 500,000 cells/ml per transwell (upper chamber). DMEM medium without FBS in the upper chamber and total DMEM medium with 10% FBS in the bottom chambers of the transwells. After culturing for 24 h, the cells from the upper chambers were removed, and the migrated cells on the undersides of the membranes were stained with crystal violet (Beyotime, Haimen, China). Migratory cells were imaged and counted in high power microscope micro-photographs (field area: 0.98 mm2) taken under bright light (Olympus Tokyo, Japan) using Image Pro Plus 6.0 software (Rockville, MD).
High power microscope micro photographs
Manufactured by Olympus
Sourced in Japan
The high power microscope micro-photographs from Olympus are precision optical instruments designed for detailed examination and imaging of small-scale specimens. They provide high-resolution, magnified views of samples, enabling users to capture detailed micro-photographs for various scientific and research applications.
Automatically generated - may contain errors
2 protocols using high power microscope micro photographs
1
Boyden Chamber Assay for Cell Migration
2
Transwell Migration Assay for Cell Motility
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Modified Boyden chamber assays were conducted using 24-well Transwell polyester membrane filter inserts with 8-μm pores and 0.33 cm2 surface area (Corning Inc., Corning, NY, USA) at a density of 500,000 cells/ml per transwell (upper chamber). The l -type calcium channel blocker verapamil (10 μM) and/or PF-573228 were used in the bottom chambers of the transwells. After culturing for 24 h, the cells from the upper chambers were removed, and the migrated cells on the undersides of the membranes were stained with crystal violet (Beyotime, Haimen, China). Migratory cells were imaged and counted in high power microscope micro-photographs (field area: 0.98 mm2) taken under bright light (Olympus Tokyo, Japan) using Image Pro Plus 6.0 software (Rockville, MD).
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