Mouse blood cell and lymphocyte development were analyzed by flow cytometry (36 (link), 37 (link)). B lymphocyte development was conducted by staining with cocktails including FITC anti-mouse CD43 (Biolegend, 553270), PE goat anti-mouse IgM (Southern Biotech, 1020-09), PE-cyanine5 anti-Hu/Mo CD45R (B220) (eBioScience, 15-0452-83), and APC anti-mouse TER119 (Biolegend, 116212). T lymphocyte development was measured on thymocytes using cocktail including PE rat anti-mouse CD4 (BD Pharmingen, 557308), FITC anti-mouse CD8a (Biolegend, 100706), PE/Cy5 anti-mouse CD3e (eBioscience, 15-0031-83), and APC anti-mouse TCRβ (BD Pharmingen, 553174). Myeloid cells were measured in bone marrow cells and splenocytes using cocktail including FITC anti-mouse CD11b (BD Pharmingen, 553310), PE rat anti-mouse CD19 (BD Pharmingen, 557399), PE/Cy5 anti-mouse CD3e (eBioscience, 15-0031-83), and APC anti-mouse Ly6G/Ly6C(Gr-1) (Biolegend, 108412). Purified splenocyte CSR was stained with PE-cyanine5 anti-Hu/Mo CD45R (B220) and FITC rat anti-mouse IgG1(BD Pharmingen, 553443). All antibodies were diluted according to the manufacturer’s protocol. The flow cytometry data were collected on either an LSR II (BD), or on an Attune NxT (Invitrogen) flow cytometer. All flow cytometry data were analyzed using FlowJo V10.
Anti mouse cd3e pe cy5
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-mouse CD3e-PE-cy5 is a fluorochrome-conjugated antibody that specifically binds to the CD3e subunit of the T cell receptor complex in mice. It can be used for the identification and enumeration of mouse T cells by flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Attune nxt, by Thermo Fisher Scientific (1 mentions)
Anti mouse cd11b fitc, by BD (1 mentions)
Fitc anti mouse cd8a, by BioLegend (1 mentions)
Pe rat anti mouse cd4, by BD (1 mentions)
Fitc rat antimouse igg1, by BD (1 mentions)
Fitc anti mouse cd43, by BioLegend (1 mentions)
Apc anti mouse ly 6g ly 6c gr 1, by BioLegend (1 mentions)
Apc anti mouse ter119, by BioLegend (1 mentions)
Pe rat anti mouse cd19, by BD (1 mentions)
Apc anti mouse tcrβ, by BD (1 mentions)
Cd4 pe, by Thermo Fisher Scientific (1 mentions)
Facscalibur, by BD (1 mentions)
Anti mouse cd4 apc, by Thermo Fisher Scientific (1 mentions)
Pe anti human cd4, by BioLegend (1 mentions)
Anti human ifn γ fitc, by BioLegend (1 mentions)
Anti human il 17a pe, by Thermo Fisher Scientific (1 mentions)
Cellquest pro software, by BD (1 mentions)
Anti mouse rat il 17a pe, by Thermo Fisher Scientific (1 mentions)
Anti human il 22 pe, by Thermo Fisher Scientific (1 mentions)
3 protocols using anti mouse cd3e pe cy5
1
Multiparametric Flow Cytometry Analysis of Immune Cell Development
2
Flow Cytometry Analysis of Splenic T Cells
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The percentages of T lymphocyte subpopulations from spleen were measured with flow cytometric analysis [20 (link)]. Splenocyte suspensions (1 × 106 cells/mL) were stained with anti-mouse CD3e-PE-cy5, CD4-PE, and CD8a-FITC antibodies (eBioscience, San Diego, CA, USA) for 20 min at room temperature in the dark. Then cells were washed with PBS to remove the excess stains. Each sample was suspended in 500 μL of PBS and analyzed with a flow cytometer (FC500, Beckman Counter, USA). The data were acquired by gating on CD3+CD4+ or CD3+CD8+ cell population.
3
Multiparametric Flow Cytometry Analysis
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Cells were stained with antibodies as indicated: anti-human IL-25-PE (R&D Systems, IC1258P), mouse IgG1 isotype control-PE (R&D Systems, IC002P), anti-human CD3-PE/Cy5 (BioLegend, 300310), anti-human CD4-PE (BioLegend, 357404), anti-human IL-17A-PE (eBioscience, 12-7179), anti-human IFN-γ-FITC (BioLegend, 502506), anti-human IL-22-PE (eBioscience, 12-7229), anti-human FOXP3-Alexa Fluor 488 (BD Pharmingen, 561181), anti-human CD274 (B1-H1)-PE (eBioscience, 12-5983), mouse IgG1 isotype control-PE (eBioscience, 12-4714), anti-human IL-25R-PE (R&D Systems, FAB1207P), mouse IgG2b isotype control-PE (R&D Systems, IC0041P), anti-mouse CD4-APC (eBioscience, 17-0041), anti-mouse CD3e-PE/Cy5 (eBioscience, 15-0031), and anti-mouse/rat IL-17A-PE (eBioscience, 12-7177). Data were collected on BD FACSCalibur (BD Biosciences) instruments and analyzed with Cell Quest Pro software (BD Biosciences).
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