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Emodin

Manufactured by Selleck Chemicals
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Sourced in United States

Emodin is a lab equipment product that serves as a chemical compound. It is a naturally occurring anthraquinone derivative that can be extracted from various plant sources.

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Lab products found in correlation

Luteolin, by Selleck Chemicals (2 mentions) Quercetin dihydrate, by Selleck Chemicals (2 mentions) Gossypol, by Selleck Chemicals (1 mentions) S2320, by Selleck Chemicals (1 mentions) Trolox, by Fujifilm (1 mentions) U0126, by Santa Cruz Biotechnology (1 mentions) L tryptophan, by Merck Group (1 mentions) Pyrimethamine, by Selleck Chemicals (1 mentions) Trichostatin a, by Merck Group (1 mentions) Phorbol 12 myristate 13 acetate, by Merck Group (1 mentions) Apigenin, by Selleck Chemicals (1 mentions) Gossypol acetate, by Selleck Chemicals (1 mentions) Caffeic acid, by Selleck Chemicals (1 mentions) Indirubin, by Selleck Chemicals (1 mentions) Kaempferol, by Selleck Chemicals (1 mentions) Myricetin, by Selleck Chemicals (1 mentions) Baicalein, by Selleck Chemicals (1 mentions) Rapamycin, by Thermo Fisher Scientific (1 mentions) Mubritinib, by Selleck Chemicals (1 mentions) Carmofur, by Selleck Chemicals (1 mentions)

7 protocols using emodin

1

Pharmacological Modulation of H9c2 Cells

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H9c2 cells (ATCC; CRL-1446) were cultured in DMEM supplemented with 10% fetal bovine serum (FBS). At 90% confluence, H9c2 cells were treated with emodin (2 hrs; SelleckChem; S2295), gossypol (2 hrs; SelleckChem; S2303), luteolin (2 hrs; SelleckChem; S2320), quercetin dihydrate (2 hrs; SelleckChem; S2347), TSA (2 hrs; Sigma T8552), or DMSO.
Godoy L.D., Lucas J.E., Bender A.J., Romanick S.S, & Ferguson B.S. (2017). Targeting the epigenome: Screening bioactive compounds that regulate histone deacetylase activity. Molecular nutrition & food research, 61(4), 10.1002/mnfr.201600744.
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2

Procurement of Reagents for Research

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Emodin and pyrimethamine were purchased from Selleck Chemicals (Houston, TX, USA), whereas l-tryptophan was from Sigma-Aldrich (St. Louis, MO, USA). Trolox (6-hydroxy-2, 5, 7, 8-tetramethylchroman-2-carboxylic acid) was obtained from Wako Pure Chemicals (Tokyo, Japan), whereas U0126 (MEK inhibitor) was from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). All reagents were of analytical grade and used as supplied unless otherwise indicated.
Adeyemi O.S., Ishii K, & Kato K. (2023). The In Vitro Anti-Parasitic Activities of Emodin toward Toxoplasma gondii. Pharmaceuticals, 16(3), 447.
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3

Emodin and Turkey Rhubarb Protocol

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Emodin was purchased from SelleckChem (S2295) and Turkey rhubarb purchased through Prescribed for Life (Sb15-H091519). Phenylephrine (PE; 10 μM) was purchased through Tocris Bioscience, phorbol-12-myristate-13-acetate (PMA; 50 nM) and trichostatin A (TSA; 200 nM) were purchased through Sigma-Aldrich. Emodin, turkey rhubarb and TSA were prepared in dimethyl sulfoxide (DMSO, Pharmco-AAPER).
Evans L.W., Bender A., Burnett L., Godoy L., Shen Y., Staten D., Zhou T., Angermann J.E, & Ferguson B.S. (2020). Emodin and emodin-rich rhubarb inhibits histone deacetylase (HDAC) activity and cardiac myocyte hypertrophy. The Journal of nutritional biochemistry, 79, 108339.
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4

Dietary Compounds from Selleckchem in Research

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The following dietary compounds were purchased from Selleckchem: apigenin (Selleckchem, Cat#- S2262), baicalein (Selleckchem, Cat#- S2269), baicalin (Selleckchem, Cat#- S2268), berberine hydrochloride (Selleckchem, Cat#- S2271), Cat#- S2268), caffeic acid (Selleckchem, Cat#- S2277), dihydromyricetin (Selleckchem, Cat#- S2399), emodin (Selleckchem, Cat#- S2295), (−)-epigallacatechin gallate (Selleckchem, Cat#- 2250), gossypol acetate (Selleckchem, Cat#- S2303), hematoxylin (Selleckchem, Cat#- S2384), indirubin (Selleckchem, Cat#- S2386), kaempferol (Selleckchem, Cat#- S2314), luteolin (Selleckchem, Cat#- S2320), morin hydrate (Selleckchem, Cat#- S2325), myricetin (Selleckchem, Cat#- S2326), myricitrin (Selleckchem, Cat#- S2327), palmatine chloride (Selleckchem, Cat#- S2397) and quercetin dihydrate (Selleckchem, Cat#- S2347).
Evans L., Shen Y., Bender A., Burnett L.E., Li M., Habibian J.S., Zhou T, & Ferguson B.S. (2021). Divergent and Overlapping Roles for Selected Phytochemicals in the Regulation of Pathological Cardiac Hypertrophy. Molecules, 26(5), 1210.
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5

Chemical Library Screening for Novel Therapeutics

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The SelleckChem library from the Molecular Screening Facility at the Wistar Institute was used for initial screens. However, once hits were identified, additional material was purchased for further testing. Mubritinib (S2216), Carmofur (S1289), Erlotinib HCl (S1023), NSC-207895 (S2678), OSI-420 (S2205), Emodin (S2295), TAK-285 (S2784), and Deguelin (S8132) were purchased from SelleckChem. Linifanib (M15676), Regorafenib (R16040), and Afatinib (G-7208) were purchased from AChemBlock. Adrucil (228440010) and Cytarabine (449561000) were purchased from Acros Organics. Lenalidomide (6305/100) and Dopamine HCl (3548/50) were purchased from Tocris Bioscience. Dacomitinib (PZ0330) and Rotenone (R8875) were purchased from Sigma. Rapamycin (J62473) was purchased from Alfa Aesar.
Calderon A., Soldan S.S., De Leo A., Deng Z., Frase D.M., Anderson E.M., Zhang Y., Vladimirova O., Lu F., Leung J.C., Murphy M.E, & Lieberman P.M. (2020). Identification of Mubritinib (TAK 165) as an inhibitor of KSHV driven primary effusion lymphoma via disruption of mitochondrial OXPHOS metabolism. Oncotarget, 11(46), 4224-4242.
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6

Drug Preparation and Cell Culture

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Emodin (Selleckchem, Houston, TX, USA) was dissolved in DMSO (dimethyl sulfoxide, Sigma-Aldrich, St. Louis, MO, USA) to a stock concentration of 10 mM and stored at −20 °C. Carfilzomib (Selleckchem, Houston, TX, USA) was dissolved in DMSO to a concentration of 100 μM. FBS (fetal bovine serum), RPMI1640 medium, L-glutamine, HEPES, sodium pyruvate, and Penicillin–Streptomycin were purchased from Gibco (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). DMSO was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Hsu C.M., Yen C.H., Wang S.C., Liu Y.C., Huang C.T., Wang M.H., Chuang T.M., Ke Y.L., Yeh T.J., Gau Y.C., Du J.S., Wang H.C., Cho S.F., Tsai Y., Hsiao C.E., Hsiao S.Y, & Hsiao H.H. (2022). Emodin Ameliorates the Efficacy of Carfilzomib in Multiple Myeloma Cells via Apoptosis and Autophagy. Biomedicines, 10(7), 1638.
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7

Purification and Characterization of Enzymes

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AmpC β-lactamase was purified from Escherichia coli as previously described.73 (link) Malate dehydrogenase (MDH) was purchased from VWR (M7032) and Sigma-Aldrich (CA97061-502). Trypsin from porcine pancreas (T0303) was purchased from Sigma-Aldrich. HIV-2 protease was kindly provided to us by Dr. Charles Craik (UCSF). CENTA (219574) was purchased from EMD Millipore. Chromogenic trypsin substrate Nα-benzoyl-dl-arginine 4-nitroanilide hydrochloride (BApNA) (B4875) was purchased from Sigma-Aldrich. Anthranilyl-HIV protease substrate (H-2992) was purchased from BACHEM. Oxaloacetate (O4126), reduced β-nicotinamide adenine dinucleotide (NADH) (N4505), brazilin (PH010674), noscapine (363960, purity: 97%), delphinidin chloride (43725, purity: ≥ 95%), kaempferol (60010, purity: ≥ 97%), and curcumin (08511, purity: ≥ 98%) were purchased from Sigma-Aldrich. Canadine (C175175, purity: 96%) and bufalin (B689510, purity: 98%) were purchased from Toronto Research Chemicals. Equol (S2450, purity: 99%), silibinin (S2357, purity: 99%), puerarin (S2346, purity: 99%), physcion (S2395, purity: 99%), phlorizin (S2343, purity: 99%), and emodin (S2295, purity: 99%) were purchased from Selleck Chemicals. Diphyllin (N038-0217) was purchased from Molport. Triton X-100 (TB0198) was purchased from Bio Basic Canada.
Duan D., Doak A.K., Nedyalkova L, & Shoichet B.K. (2015). Colloidal Aggregation and the in Vitro Activity of Traditional Chinese Medicines. ACS chemical biology, 10(4), 978-988.
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