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Keap1

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Keap1 is a protein that plays a crucial role in the regulation of cellular stress response pathways. It functions as a sensor for oxidative and electrophilic stress, and its primary role is to modulate the activity of the transcription factor Nrf2, which is responsible for the expression of genes involved in antioxidant and detoxification systems.

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Lab products found in correlation

Ubiquitin, by Merck Group (2 mentions) β actin, by Abcam (2 mentions) Digoxigenin, by Merck Group (1 mentions) Digoxin, by Merck Group (1 mentions) Rapamycin, by Merck Group (1 mentions) Lamp1, by Abcam (1 mentions) Protein a magnetic beads, by Thermo Fisher Scientific (1 mentions) Dapi mounting media, by Merck Group (1 mentions) Flag tag (flag), by OriGene (1 mentions) Caspase 3, by Santa Cruz Biotechnology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) N acetylcysteine, by Merck Group (1 mentions) Actinomycin d, by Merck Group (1 mentions) Arachidonic acid, by Cayman Chemical (1 mentions) Propidium, by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) Sphingosine 1 phosphate (s1p), by Avanti Polar Lipids (1 mentions) Sphingosine 1 phosphate (s1p), by Enzo Life Sciences (1 mentions) Fty720, by Echelon Biosciences (1 mentions) Dihydro s1p, by Enzo Life Sciences (1 mentions)

2 protocols using keap1

1

Autophagy and Apoptosis Regulation

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Chloroquine, ammonium chloride, digoxin, digoxigenin, strophanthidin, DAPI mounting media, rapamycin, 3‐(4,5‐dimethylthiazol‐2yr)‐2‐5‐diphenyltetrazoliumbromide (MTT), actinomycin D, bafilomycin SMER28 and N‐acetylcysteine from Sigma (Poole, UK) were used.
Protein A magnetic beads were purchased from Thermo Scientific (East Riding of Yorkshire, UK). Antibodies against the following were used for immunoblotting: p62 (rabbit polyclonal; #P0067), LC3 (rabbit polyclonal; #L8918), Ubiquitin (rabbit polyclonal; #SAB1306222), NBR1 (rabbit polyclonal, #SAB2107031) from Sigma. p62 (mouse monoclonal; # sc‐28359), caspase‐3 (rabbit polyclonal; #sc‐136219) from Santa Cruz Biotechnology. Beta‐actin (mouse monoclonal; #ab8226), GFP (#ab1218), and LAMP1 (rabbit polyclonal; #ab24170) from Abcam. Cleaved caspase‐3 (rabbit polyclonal; #9661) from Cell Signalling (New England Biolabs). Keap1 (mouse monoclonal; #TA502059) and FLAG (mouse monoclonal; #TA50011‐100) from Origene).
Mercado N., Colley T., Baker J.R., Vuppussetty C., Kono Y., Clarke C., Tooze S., Johansen T, & Barnes P.J. (2019). Bicaudal D1 impairs autophagosome maturation in chronic obstructive pulmonary disease. FASEB bioAdvances, 1(11), 688-705.
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2

Sphingolipids and Antioxidant Pathways

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Sphingosine, SKI-II (2-(p-Hydroxyanilino)-4-(p-chlorophenyl) thiazole), DMS (N,N-Dimethylsphingosine), DHS (dihydro-sphingosine ), FTY720 and SK1-I ((2R,3S,4E)-N-methyl-5-(4′-pentylphenyl)-2-aminopent-4-ene-1,3-diol) were obtained from Echelon Biosciences (Salt Lake City, UT). Hydrogen peroxide,3-(4,5-dimethylthiazol-2 yr)-2-5-diphenyl tetrazoliumbromide (MTT)sulforaphane,CDDO-Imidazolide and propidium were from Sigma (Poole, UK). Dihydrospingosine was from Tocris Bioscience (Bristol, UK). S1P, dihydroS1P and fumonisin B1 were from Enzo Life Sciences International (Exeter, UK). C17s sphingosine, dihydrosphyngosine, S1P and dihydroS1P were purchased from Avanti Polar Lipids (Alabaster, AL). FITC-conjugated anti-macrophage (MOMA2) antibody and anti-neutrophils (7/4) antibody were purchased from Acris Antibodies (GmbH, Herford, Germany). Arachidonic acid and Biotin-Arachidonic acid were purchased from Cayman (Ann Arbor, MI). Neutravidin Agarose Resin was purchased from Thermo Scientist (East Riding of Yorkshire, UK). Antibodies against the following were used for immunoblotting: β-actin, p62, NQO1, ubiquitin (Sigma), Nrf2 (Santa Cruz Biotechnology, Santa Cruz, CA), Nrf2, GCLM, HO-1, TBP, β-actin, SK2 (Abcam, Cambridge, UK), SK1 (Abgent, San Diego, CA) and Keap1 (Origene, Rockville, MD).
Mercado N., Kizawa Y., Ueda K., Xiong Y., Kimura G., Moses A., Curtis J.M., Ito K, & Barnes P.J. (2014). Activation of Transcription Factor Nrf2 Signalling by the Sphingosine Kinase Inhibitor SKI-II Is Mediated by the Formation of Keap1 Dimers. PLoS ONE, 9(2), e88168.
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