Sodium alginate with high (232 kDa) and medium (139 kDa) molecular weight and high guluronic acid content was purchased from FMC Biopolymer (Princeton, NJ). To produce the low molecular weight alginate, the high molecular weight alginate was gamma-irradiated with a cobalt-60 source at a gamma-dose of 3 Mrad. Alginates were covalently coupled with the integrin-binding peptide (Gly)4-Arg-Gly-Ala-Ser-Ser-Lys-Tyr (Peptides International) and either Fluoresceinamine, isomer I (Sigma-Aldrich) or LissamineTM Rhodamine B Ethylenediamine (Setareh Biotech). 1-bicyclo[2.2.1]hept-5-en-2-ylmethanamine (norbornene; Matrix Scientific) or 3-(p-benzylamino)-1,2,4,5-tetrazine (Sigma Aldrich) were prepared and conjugated to 232-kDa alginate as described previously23 .
Gly 4 arg gly ala ser ser lys tyr
Manufactured by Peptide Institute
The (Gly)4-Arg-Gly-Ala-Ser-Ser-Lys-Tyr is a peptide sequence composed of the amino acids glycine, arginine, alanine, serine, lysine, and tyrosine. It serves as a lab equipment product for research and analysis purposes.
Automatically generated - may contain errors
2 protocols using gly 4 arg gly ala ser ser lys tyr
1
Alginate Biopolymer Functionalization for Cell Culture
2
Alginate Biopolymer Functionalization for Cell Culture
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Sodium alginate with high (232 kDa) and medium (139 kDa) molecular weight and high guluronic acid content was purchased from FMC Biopolymer (Princeton, NJ). To produce the low molecular weight alginate, the high molecular weight alginate was gamma-irradiated with a cobalt-60 source at a gamma-dose of 3 Mrad. Alginates were covalently coupled with the integrin-binding peptide (Gly)4-Arg-Gly-Ala-Ser-Ser-Lys-Tyr (Peptides International) and either Fluoresceinamine, isomer I (Sigma-Aldrich) or LissamineTM Rhodamine B Ethylenediamine (Setareh Biotech). 1-bicyclo[2.2.1]hept-5-en-2-ylmethanamine (norbornene; Matrix Scientific) or 3-(p-benzylamino)-1,2,4,5-tetrazine (Sigma Aldrich) were prepared and conjugated to 232-kDa alginate as described previously23 .
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