Total CD4+ T cells from spleens of MRL/lpr mice were purified with negative selection using a Mouse T Cell Isolation Kit (Stem Cell Technologies Inc) according to the manufacturer’s instructions. The purity was determined by FACS analysis (>98% CD4+). For T cell differentiation, the above purified naïve CD4+ T cell were cultured in RPMI-1640 with 10% heat-inactivated FBS and 2 mM L-glutamine in the presence of 1 μg/mL anti-mouse CD3 (Biolegend) and 0.5 μg/mL anti-mouse CD28 (Biolegend), followed by treatment with or without NCTD. Then, 2 ng/mL transforming growth factor-1 (TGF-β1) (Peprotech), 20 ng/mL IL-6 (Peprotech), 10 μg/mL anti-IL-4 antibody (Biolegend) and 10 μg/mL anti-IFN-γ antibody (Biolegend) were added to drive Th17 cell polarization. After 4 days, cells were collected for FACS analysis and cell culture supernatants were also collected to detect the IL-17 production as described above.
Transforming growth factor 1 tgf β1
Manufactured by Thermo Fisher Scientific
Sourced in United States
Transforming growth factor-1 (TGF-β1) is a protein that plays a key role in the regulation of cell growth, differentiation, and other cellular processes. It is a member of the transforming growth factor beta family of cytokines. TGF-β1 is involved in a variety of biological and physiological functions, including cell proliferation, immune response, and tissue repair.
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Lab products found in correlation
Anti ifn γ antibody, by BioLegend (2 mentions)
Anti il 4 antibody, by BioLegend (2 mentions)
Mouse t cell isolation kit, by STEMCELL (2 mentions)
Interleukin 6 (il 6), by Thermo Fisher Scientific (2 mentions)
Anti mouse cd3, by BioLegend (1 mentions)
Anti mouse cd28, by BioLegend (1 mentions)
Anti cd3, by BioLegend (1 mentions)
Anti cd28, by BioLegend (1 mentions)
2 protocols using transforming growth factor 1 tgf β1
1
Th17 Cell Differentiation from Naive CD4+ T Cells
2
Th17 Cell Differentiation from Murine CD4+ T Cells
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Total CD4 T cells from spleens of MRL/lpr mice were purified with negative selection using a Mouse T Cell Isolation Kit (Stem Cell Technologies Inc) according to the manufacturer's instructions. The purity was determined by FACS analysis (>98% CD4+). For T cell differentiation, the above purified naïve CD4+ T cell were cultured in RPMI 1640 with 10% heat-inactivated FBS and 2 mM L-glutamine in the presence of 1 μg/ml anti-CD3 (Biolegend) and 0.5 μg/ml anti-CD28 (Biolegend), followed by treatment with or without NCTD. Then, 2 ng/ml transforming growth factor-1(TGF-β1) (Peprotech, Rocky Hill, NJ, USA), 20 ng/ml IL-6 (Peprotech), 10 μg/ml anti-IL-4 antibody (Biolegend) and 10 μg/ml anti-IFN-γ antibody (Biolegend) were added to drive Th17 cell polarization. After 4 days, cells were collected for FACS analysis and cell culture supernatants were also collected to detect the IL-17A production as described above.
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