Cells were stained in HEPES buffer + 0.5% BSA (25–30 min, 4 °C), measured using a BD FACSCanto™ II flow cytometer (BD Biosciences), gated against specific isotypes, and analyzed using FlowJo™ (version 10.3; USA). Antibodies or reagents used were: (i) CD235a-PE (1:2500 dilution; OriGene cat#DM066R), CD49d-BV421 (1:100 dilution; BD-Biosciences cat#565,277), DRAQ7 (live/dead stain; 1:200 dilution; ThermoFischer Scientific cat#D15106); (ii) CD235a-PE (1:2500 dilution; OriGene cat#DM066R), CD71-APC (1:200 dilution; Miltenyi cat#130-d099-219); (iii) CD71-VioBlue (1:200 dilution; Miltenyi cat#130-101-631); (iv) DRAQ5 (nuclear stain; 1:2500 dilution; abcam cat#ab108410); (v) PI (live/dead stain; 1:2000 dilution; Invitrogen cat#P3566).
Cd49d bv421
Manufactured by BD
CD49d-BV421 is a fluorochrome-conjugated antibody that binds to the CD49d cell surface antigen. CD49d, also known as the alpha-4 subunit of the VLA-4 integrin, is expressed on various cell types including lymphocytes, monocytes, and eosinophils. The BV421 fluorochrome is used to label the antibody, allowing for its detection and quantification by flow cytometry.
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Lab products found in correlation
2 protocols using cd49d bv421
1
Multiparametric Flow Cytometry Analysis
2
Multiparametric Flow Cytometry Staining Protocol
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About 200,000 cells were stained in 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid buffer + 0.5% bovine serum albumin (30 min, 4°C), measured using an BD FACSCanto™ II or Accuri C6 flow cytometer (BD Biosciences), gated against specific isotypes, and analyzed using FlowJo™ (version 10.3). Antibodies or reagents used were: (i) CD235a‐PE (1:2500 dilution; OriGene cat#DM066R), CD49d‐BV421 (1:100 dilution; BD‐Biosciences cat#565277), DRAQ7 (live/dead stain; 1:200 dilution; Thermo Fischer Scientific cat#D15106); (ii) CD235a‐PE (1:2500 dilution; OriGene cat#DM066R), CD71‐APC (1:200 dilution; Miltenyi cat#130‐099‐219); (iv) propidium iodide (PI) (live/dead stain; 1:2000 dilution; Invitrogen cat#P3566); (v) AnnexinV‐FITC (1:1000 dilution; BioLegend cat#640906), DRAQ7 (live/dead stain; 1:200 dilution; Thermo Fischer Scientific cat#D15106); (vi) DRAQ5 (nuclear stain; 1:2500 dilution; abcam cat# ab108410). Stainings with panels (iv), (v), and (vi) were performed 10 min before analysis without intermediate washing steps.
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