Pyoverdine in the supernatants of cultures grown for 16 h at 30°C and 1000 rpm in 96-well microtiter plates containing glucose E2 medium was quantified with an Infinite 500 Plate Reader (Tecan, Crailsheim) by measuring fluorescence emission at 485 nm after excitation at 420 nm. The values were expressed relative to the OD600 of the corresponding cultures.
Infinite 500 plate reader
Manufactured by Tecan
The Infinite 500 Plate Reader is a multi-mode microplate reader capable of absorbance, fluorescence, and luminescence detection. It provides reliable and accurate measurement of various biological assays in a 96-well or 384-well microplate format.
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Lab products found in correlation
3 protocols using infinite 500 plate reader
1
Quantification of Pyoverdine Production
2
Quantification of PVD using Infinite 500
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Infinite 500 Plate Reader (Tecan, Crailsheim) was used to quantify PVD in the supernatants of the cultures by measuring fluorescence emission at 485 nm after excitation at 420 nm.
3
Fluorescent Tracer Diffusion Assay
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Compartments were filled with 50
μL of either 5 μM fluorescein (F7137, Sigma), 62.5 μg/mL
70 kD fluorescein-labeled dextran (D1823, Invitrogen), or fluorescein-labeled
beads with 40 nm (0.025%, F10720 Invitrogen), respectively, 500 nm
diameter (0.008%, F8813, Invitrogen). Adjacent compartments were filled
with either equal volumes or twice the volume to establish a direct
flow. Plates were sealed and imaged for 72 h on an Infinite 500 plate
reader (Tecan) at 485/535 nm (excitation/emission).
μL of either 5 μM fluorescein (F7137, Sigma), 62.5 μg/mL
70 kD fluorescein-labeled dextran (D1823, Invitrogen), or fluorescein-labeled
beads with 40 nm (0.025%, F10720 Invitrogen), respectively, 500 nm
diameter (0.008%, F8813, Invitrogen). Adjacent compartments were filled
with either equal volumes or twice the volume to establish a direct
flow. Plates were sealed and imaged for 72 h on an Infinite 500 plate
reader (Tecan) at 485/535 nm (excitation/emission).
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