Quantitative elisa kit

Manufactured by Thermo Fisher Scientific

Quantitative ELISA (Enzyme-Linked Immunosorbent Assay) kits are laboratory tools designed to measure the concentration of specific target analytes in samples. These kits utilize the principle of antigen-antibody interactions to detect and quantify the presence of the target molecule. The kits provide a standardized and reproducible approach to quantitative analysis, offering researchers a reliable method for measuring the levels of proteins, hormones, cytokines, and other biomolecules in a variety of sample types.

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Lab products found in correlation

Luminex assay kit, by R&D Systems (2 mentions)

Close spelling variants of this product

Specific quantitative sandwich ELISA kits Human soluble c-Met quantitative ELISA kit Quantitative ELISA ELISAs

5 protocols using quantitative elisa kit

Cytokine and Chemokine Profiling

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IL-1β was quantified with a quantitative ELISA kit, according to the manufacturer’s instructions (Invitrogen, Thermo Fisher). The levels of mouse CCL2, CCL3, CXCL1, CXCL2, EGF, FGF basic, IFNγ, IGF-1, IL-1β, IL-6, IL-10, IL-12p70, IL-13, LIX, MMP-9, oncostatin M, TIMP-1, TNFα and VEGF were determined with a Luminex assay kit (R&D Systems).

Foulon M., Robbe-Saule M., Manry J., Esnault L., Boucaud Y., Alcaïs A., Malloci M., Fanton d’Andon M., Beauvais T., Labarriere N., Jeannin P., Abel L., Saint-André J.P., Croué A., Delneste Y., Boneca I.G., Marsollier L, & Marion E. (2020). Mycolactone toxin induces an inflammatory response by targeting the IL-1β pathway: Mechanistic insight into Buruli ulcer pathophysiology. PLoS Pathogens, 16(12), e1009107.

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Quantitative Cytokine Profiling

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IL-1β, IL-6 and IFN-β were quantified with a quantitative ELISA kit, according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific). The levels of mouse CXCL1, CCL3, IL1α, IL1β TNFα, IL6, CCL2, IL33, CXCL2, IL10, TMP-1 and VEGF were determined with a Luminex assay kit (R&D Systems).

Bernard Q., Goumeidane M., Chaumond E., Robbe-Saule M., Boucaud Y., Esnault L., Croué A., Jullien J., Marsollier L, & Marion E. (2023). Type-I interferons promote innate immune tolerance in macrophages exposed to Mycobacterium ulcerans vesicles. PLOS Pathogens, 19(7), e1011479.

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Cytokine Quantification in Cell Cultures

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Levels of IL-1β, IL-6, and TNF-α obtained from cell culture after stimulations and mice blood serum were detected in quantitative ELISA kits (eBioscience), according to the manufacturer’s instructions.

Shi J., Xia Y., Wang H., Yi Z., Zhang R, & Zhang X. (2022). Piperlongumine Is an NLRP3 Inhibitor With Anti-inflammatory Activity. Frontiers in Pharmacology, 12, 818326.

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Cytokine Profiling in Cell Culture and Sera

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Levels of IL-1β, TNF-α and IL-6 collected from cell culture and sera were determined using quantitative ELISA kits (eBioscience) according to the manufacturer’s instructions.

Zhang Y., Luo L., Xu X., Wu J., Wang F., Lu Y., Zhang N., Ding Y., Lu B, & Zhao K. (2023). Acetylation is required for full activation of the NLRP3 inflammasome. Nature Communications, 14, 8396.

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Quantitative Cytokine Profiling

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Levels of IL-1β, TNF-α, and IL-18 collected from cell culture and sera were determined using quantitative ELISA kits (eBioscience or abcam) according to the manufacturer’s instructions.

Wang D., Zhang Y., Xu X., Wu J., Peng Y., Li J., Luo R., Huang L., Liu L., Yu S., Zhang N., Lu B, & Zhao K. (2021). YAP promotes the activation of NLRP3 inflammasome via blocking K27-linked polyubiquitination of NLRP3. Nature Communications, 12, 2674.

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