Non target control lentiviral vectors

pMD-VSV-G and delta 8.2 plasmids were gifts from Dr. Dong at Tulane University. Plasmid maps are shown in Supplementary Figure 2. EGFR and non-target control lentiviral vectors were purchased from Sigma-Aldrich. Transfection was performed using Lipofectamine 3000 reagent (Invitrogen, USA) according to the manufacturer’s instructions. Briefly, a lentivirus vector, pMD-VSV-G, and delta 8.2 were cotransfected into TLA-HEK293T cells, and the culture medium was harvested 72 h post-transfection. BxPC-3 and HPAC cells were transduced by adding virus supernatant and polybrene (Sigma-Aldrich) for 3 h. Cells were harvested 5 days after lentiviral infection and used for subsequent analysis.