The cell culture medium was serially diluted in EMEM containing 2% FBS and was added to 4 × 104 Vero E6 cells plated on 96-well plates. After incubation for 8 h at 37°C, cells were washed and fixed with a 4% formaldehyde solution. Cells were stained with the anti-SARS-CoV-2 NP antibody (40143-R001, Sino Biological, Beijing, China) and a secondary horseradish peroxidase-conjugated goat anti-rabbit IgG (Bio-Rad, Hercules, CA). The signal was developed using an insoluble tetramethylbenzidine (TMB) substrate (Promega, Madison, WI, USA), and the number of infected cells was counted using an ImmunoSpot reader (CTL, Shaker Heights, OH).
Anti sars cov 2 np antibody
Manufactured by Sino Biological
Sourced in China
The Anti-SARS-CoV-2 NP antibody is a laboratory reagent that specifically binds to the nucleoprotein (NP) of the SARS-CoV-2 virus. It can be used in various immunoassay applications to detect the presence of SARS-CoV-2 NP in samples.
Automatically generated - may contain errors
2 protocols using anti sars cov 2 np antibody
1
SARS-CoV-2 Neutralization Assay in Vero E6 Cells
2
SARS-CoV-2 Neutralization Assay in Vero E6 Cells
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The cell culture medium was serially diluted in EMEM containing 2% FBS and was added to 4 × 104 Vero E6 cells plated on 96-well plates. After incubation for 8 h at 37°C, cells were washed and fixed with a 4% formaldehyde solution. Cells were stained with the anti-SARS-CoV-2 NP antibody (40143-R001, Sino Biological, Beijing, China) and a secondary horseradish peroxidase-conjugated goat anti-rabbit IgG (Bio-Rad, Hercules, CA). The signal was developed using an insoluble tetramethylbenzidine (TMB) substrate (Promega, Madison, WI, USA), and the number of infected cells was counted using an ImmunoSpot reader (CTL, Shaker Heights, OH).
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