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Biacore 8k spr system

Manufactured by Cytiva
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Sourced in United States

The Biacore 8K SPR system is a label-free and real-time biomolecular interaction analysis instrument. It utilizes surface plasmon resonance (SPR) technology to detect and analyze interactions between molecules in a sample and a surface-immobilized target molecule. The system provides quantitative kinetic and affinity data on a wide range of biomolecular interactions.

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Lab products found in correlation

Series s sensor chip cm5, by Cytiva (1 mentions) Series s sa sensor chips, by Cytiva (1 mentions) Biacore evaluation software, by Cytiva (1 mentions) Prism 9, by GraphPad (1 mentions) Hbs ep, by Cytiva (1 mentions)

Close spelling variants of this product

BIAcore 8K system Biacore 8K

3 protocols using biacore 8k spr system

1

Determining DuAb and EDuAb Binding Kinetics

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To determine the binding kinetics and affinity constants of DuAb and EDuAb with CD3 and CD19 proteins, we used an optical biosensor based on surface plasmon resonance (SPR) detection. A Biacore 8 K SPR system (Cytiva, USA) equipped with series S Sensor Chip CM5 was used to generate binding kinetic rate and affinity constants at 25 °C in a running buffer. The CM5 chip was activated and coated with human CD3E & CD3D heterodimer protein (Acro, China) and human CD19 protein (Acro, China) to detect the reactions between DuAb or EDuAb with CD3 and CD19 proteins, respectively. We followed the detailed protocol provided by Acro company. The binding kinetics and affinity constants were then generated based on the data obtained from the SPR system.
Chen M., Liu X., Peng N., Zhang T., Mou J., He H., Wang Y., Xu Y., Xing H., Tang K., Tian Z., Rao Q., Gu R., Qiu S., Wang M, & Wang J. (2023). Construction of CD19 targeted dual- and enhanced dual-antibodies and their efficiency in the treatment of B cell malignancy. Experimental Hematology & Oncology, 12, 64.
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2

SPR-based Kinetic Analysis of Protein-Compound Interactions

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SPR experiments were carried out at 25 °C on a Biacore 8K SPR system (Cytiva, USA). Series S SA sensor chips from Cytiva were used in all experiments. All experiments were run in an identical buffer made of 20 mM Tris/HCl pH 7.0, 150 mM NaCl, 0.005% Tween20 (v/v), and 2 mM DTT. Biotinylated protein was first immobilized on the SA chip (contact time: 60 s, flow rate: 10 μL/min) prior to flowing varying concentrations of compound over the immobilized protein in a dose–response fashion (contact time: 120 s, dissociation time: 300 s, flow rate: 30 μL/min) using a multicycle kinetics protocol. The resulting data was analyzed using Biacore Evaluation Software (Cytiva, USA). GraphPad Prism 9.0 was used to plot the analyzed data for the final figure preparation.
Shell D.J., Foley C.A., Wang Q., Smith C.M., Guduru S.K., Zeng H., Dong A., Norris-Drouin J.L., Axtman M., Hardy P.B., Gupta G., Halabelian L., Frye S.V., James L.I, & Pearce K.H. (2023). Discovery of a 53BP1 Small Molecule Antagonist Using a Focused DNA-Encoded Library Screen. Journal of medicinal chemistry, 66(20), 14133-14149.
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3

LEDGF-PWWP SPR Binding Experiments

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LEDGF-PWWP SPR experiments were carried out on a Biacore 8K SPR system (Cytiva, USA). Biotinylated protein was immobilized on the flow cell of an SA sensor chip in 1X HBS-EP (Cytiva, USA) buffer, yielding approximately 4300 RU. Using the same buffer, with 0.5% (replicate 1) or 2% (replicate 2) of DMSO, single-cycle kinetics were used with a 60 sec contact time and a dissociation time of 120 sec at a flow rate of 75 μL/min. Compounds were tested at 100 μM (replicate 1) or 200 μM (replicate 2) as the highest concentration. A dilution factor of 0.25 was used to yield 5 concentrations for testing.
Shell D.J., Rectenwald J.M., Buttery P.H., Johnson R.L., Foley C.A., Guduru S.K.R., Uguen M., Rubiano J.S., Zhang X., Li F., Norris-Drouin J.L., Axtman M., Brian Hardy P., Vedadi M., Frye S.V., James L.I, & Pearce K.H. (2022). Discovery of hit compounds for methyl-lysine reader proteins from a target class DNA-encoded library. SLAS discovery : advancing life sciences R & D, 27(8).
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