The AlphaLISA® Human Interleukin-6 (IL6) Detection Kit (Perkin Elmer Inc.) was used to measure IL6 in CM following the manufacturer's instructions. To prepare conditioned media (CM), cells were cultured in serum-depleted media overnight, unless indicated. IL6 levels were normalized the number of cells in the plate. For ELISA, CM was prepared as follows: cells were washed with 1× PBS twice, then incubated with fresh DMEM with 10% FBS. After incubation for an additional 24-48 h, the CM was collected for further analysis. IL6 secreted into the culture medium were measured using a Human IL6 Quantikine ELISA Kit (R&D systems).
Alphalisa human interleukin 6 il6 detection kit
Manufactured by PerkinElmer
The AlphaLISA® Human Interleukin-6 (IL6) Detection Kit is a bead-based assay used to quantitatively measure the concentration of human interleukin-6 (IL-6) in biological samples, such as cell culture supernatants and serum. The kit utilizes AlphaLISA technology to detect and quantify the target analyte.
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2 protocols using alphalisa human interleukin 6 il6 detection kit
1
Quantifying Interleukin-6 Secretion in Cell Culture
2
Quantifying Interleukin-6 in Cell Culture
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The AlphaLISA® Human Interleukin-6 (IL6) Detection Kit (Perkin Elmer Inc.) was used to measure IL6 in conditioned media (CM) according to the manufacturer's instructions. To prepare CM, cells were cultured in serum-depleted media for 18 h, unless indicated. IL6 levels were normalized to cell numbers in the population.
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