Bapta am 2787

Manufactured by Bio-Techne

BAPTA-AM (2787) is a cell-permeable calcium chelator that can be used to study calcium-mediated signaling processes in biological systems. It functions by rapidly binding and sequestering intracellular calcium ions.

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Lab products found in correlation

Mouse anti actin ab3280, by Abcam (1 mentions) Bortezomib s1013, by Selleck Chemicals (1 mentions) Cytochalasin d c8273, by Merck Group (1 mentions) Kn 62, by Selleck Chemicals (1 mentions) Yoda1 5586, by Bio-Techne (1 mentions) Gtpγs g8634, by Merck Group (1 mentions) Lps l4391, by Merck Group (1 mentions)

2 protocols using bapta am 2787

CRISPR Targeting of ATP2C1 Gene

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Guide RNAs against ATP2C1 were designed and ordered as a single-stranded oligonucleotide from IDT (5′-GGAGCTGTCACCTTAGAACA-3′); the scrambled control guide (Scr; 5′-GGTCTCTGTACGGGCCGCCC-3′) does not align with the human genome by primer blast. Following phosphorylation and annealing, guides were ligated into BsmBI-digested LentiCrisprV2 (a gift from the Feng Zhang lab; Addgene plasmid number 52961). Mouse anti-actin (ab3280) was obtained from Abcam (Cambridge, MA), while goat-anti-mouse horseradish peroxidase (HRP) (32430) was obtained from Thermo Fisher. Rabbit anti-lamin B1 (12586) was obtained from Cell Signaling Technology (Danvers, MA). Hybridoma AAV antibodies A1, A69, and B1 were purchased from American Research Products, Inc. (Waltham, MA). Fluo4-am (no. F14201) was purchased from Thermo Fisher. Ionomycin (2092) and BAPTA-AM (2787) were obtained from Tocris Bioscience (Minneapolis, MN). Bortezomib (S1013) was obtained from Selleck Chemicals (Houston, TX).

Madigan V.J., Berry G.E., Tyson T.O., Nardone-White D., Ark J., Elmore Z.C., Murlidharan G., Vincent H.A, & Asokan A. (2020). The Golgi Calcium ATPase Pump Plays an Essential Role in Adeno-associated Virus Trafficking and Transduction. Journal of Virology, 94(21), e01604-20.

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Biochemical Modulators of Cell Signaling

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LPS (L4391), GTPγS (G8634), and Cytochalasin D (C8273) were from Sigma-Aldrich. Bisindolylmaleimide I (S7208), TAE226 (S2820), and KN-62 (S7422) were from Selleck. TFA (P0214A) was from MedChemExpress (MCE). BAPTA-AM (2787) and Yoda1 (5586) were from TOCRIS. The Phos-tag TM Acrylamide AAL-107 was from the NARD Institute.

Geng J., Shi Y., Zhang J., Yang B., Wang P., Yuan W., Zhao H., Li J., Qin F., Hong L., Xie C., Deng X., Sun Y., Wu C., Chen L, & Zhou D. (2021). TLR4 signalling via Piezo1 engages and enhances the macrophage mediated host response during bacterial infection. Nature Communications, 12, 3519.

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