Uv vs spectrophotometer 117

Fresh leaf samples and flower buds were collected one month before flower bud differentiation and at the time of fruit bud differentiation, respectively. For this, terminal shoots were tagged in July, and leaves were harvested after 4 months of growth for recording biochemical traits and nutrient acquisition, while buds were collected in December. A total of 30 leaves /samples (3^rd and 4^th mature leaves from the top of the terminal shoot) were collected for sampling for nutrient observations. For estimation of P, K, Na, Ca, Mg, S, and micronutrients B, Fe, Mn, Cu, and Zn, one gram of the plant sample was digested in 4:1 nitric acid and perchloric acid (HNO₃: HClO₄) mixture. Phosphorus (%) (Vanadomolybdophosphoric yellow color method) [25 ] with spectrophotometer (Systronics UV- VS Spectrophotometer 117), Potassium (%) and Sodium with Flame photometer (Systronics Flame Photometer 128) [25 ] and Sulphur (%) (Turbidimetric method) [26 (link)] with spectrophotometer (Systronics UV- VS Spectrophotometer 117) estimated. Calcium (%), Magnesium (%) [25 ], and micronutrients- Fe, Mn, Cu, Zn (ppm) [27 (link)] with Motras Scientific Atomic Absorption Spectrophotometer recorded. Boron (ppm) estimated with Azomethine-H [28 (link)] with spectrophotometer- [Systronics UV- VS Spectrophotometer 117].