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Countess cell counting machine

Manufactured by Thermo Fisher Scientific
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The Countess cell counting machine is a compact and automated device designed to provide accurate cell counts. It uses advanced imaging technology to capture and analyze cell samples, delivering reliable and consistent results.

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Lab products found in correlation

Lineage depletion kit, by Miltenyi Biotec (2 mentions) Cxcl12, by Thermo Fisher Scientific (2 mentions)

2 protocols using countess cell counting machine

1

Assaying Chemotactic Migration of Murine Bone Marrow Cells

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Fresh BM cells were isolated from mutant mice and from control animals, and lineage-positive cells were depleted using magnetic separation (lineage depletion kit, Miltenyi Biotec) and were resuspended in PBS containing 0.5% BSA. Lineage-negative cells (0.5–1 million) were pretreated for 3 h at 37 °C with the p110β-specific inhibitor KIN193 (1 μM), NSC23766 (50 μM) PTX from Bordetella pertussis (100 ng ml−1) or dimethylsulphoxide (DMSO; 1:1,000), or directly loaded (105 cells in 100 μl) in the top chamber of a 6.5-mm diameter 8-μm pore polycarbonate Transwell insert (Corning Costar); inserts with cells were then placed in wells containing CXCL12 (50 mM, PeproTech), and incubated for 3 h at 37 °C. Cells migrating from the top to the bottom chamber were collected and quantified using a Countess cell counting machine (Invitrogen).
Yuzugullu H., Baitsch L., Von T., Steiner A., Tong H., Ni J., Clayton L.K., Bronson R., Roberts T.M., Gritsman K, & Zhao J.J. (2015). A PI3K p110β–Rac signalling loop mediates Pten-loss-induced perturbation of haematopoiesis and leukaemogenesis. Nature Communications, 6, 8501.
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2

Chemotactic Migration of Lineage-Depleted Cells

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Fresh BM cells were isolated from mutant mice and from control animals and Lineage positive cells were depleted using magnetic separation (Lineage depletion kit, Miltenyi Biotec), and were resuspended in PBS containing 0.5% BSA. Lineage negative cells (0.5–1mio) were pretreated for 3h at 37 °C with the p110β specific inhibitor KIN193 (1μM), NSC23766 (50uM) pertussis toxin from Bordetella Pertussis (PTX, 100 ng/mL), or DMSO (1:1000), or directly loaded (105 cells in 100μl) in the top chamber of a 6.5-mm diameter 8μm pore polycarbonate transwell insert (Corning Costar); inserts with cells were then placed in wells containing CXCL12 (50mM, Peprotech), and incubated for 3h at 37 °C. Cells migrating from the top to the bottom chamber were collected and quantified using a Countess Cell Counting Machine (Invitrogen).
Yuzugullu H., Baitsch L., Von T., Steiner A., Tong H., Ni J., Clayton L.K., Bronson R., Roberts T.M., Gritsman K, & Zhao J.J. (2015). A PI3K p110β–Rac signalling loop mediates Pten-loss-induced perturbation of haematopoiesis and leukaemogenesis. Nature Communications, 6, 8501.
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