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Mouse monoclonal anti gfp 1814460

Manufactured by Roche
Sourced in Switzerland

Mouse monoclonal anti-GFP (1814460) is a laboratory reagent that binds to green fluorescent protein (GFP). It is intended for use in various applications that involve the detection and analysis of GFP-labeled proteins or cells.

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2 protocols using mouse monoclonal anti gfp 1814460

1

Antibody Usage for Protein Detection

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Used primary antibodies were used: mouse monoclonal anti-GFP (1814460; Roche Diagnostics, Basel, Switzerland); polyclonal serum (rabbit) recognizing the mitogen-activated protein kinase (MAPK) phosphorylated TEY motif (V8031; Promega, Madison, WI)13 (link); sheep polyclonal anti-phosphoTyr171 CDKL522 (link) and sheep polyclonal anti-phosphoSer900 MAP1S22 (link) (both obtained from MRC-PPU Reagents, University of Dundee, Dundee, United Kingdom); monoclonal anti-CDKL5 (mouse; sc-376314; Santa Cruz Biotechnologies, Dallas, TX); and polyclonal anti-RFP (rabbit; PM005; MBL International, Woburn, MA).
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2

Antibodies for Cellular Protein Analysis

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The following antibodies were used for western blot and immunofluorescence experiments: mouse monoclonal anti-CDKL5 (for western blot) (sc-376314; Santa-Cruz Biotechnologies, Dallas, TX, USA), rabbit polyclonal anti-CDKL5 (for immunofluorescence) (HPA002847; Sigma-Aldrich, St. Louis, MO, USA), rabbit polyclonal anti-active Mitogen-Activated Protein Kinase (MAPK; used to detect the phosphorylated TEY motif; V8031, Promega, Madison, WI, USA), mouse monoclonal anti-α-tubulin (T6074; Sigma-Aldrich), mouse monoclonal anti-GFP (1814460; Roche Diagnostics, Basel, Switzerland), mouse monoclonal anti-GAPDH (G3171; Sigma-Aldrich).
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