5 fluro 2 deoxyuridine fudr

All strains were grown on NGM plates for 2-3 generations without starvation. All lifespan assays were performed at 20 °C except CF1903, according to standard protocols and as previously described [53 (link)]. In brief, 100 late L4 larvae or young adults were transferred to fresh plates containing 10 μM 5-fluro-2′-deoxyuridine (FUdR, Sigma) and the respective compounds and scored every day. To determine the lifespan of the temperature-sensitive mutant CF1903, L1 worms were incubated at 20 °C for 12 h, then transferred to 25 °C until L4 larvae or young adulthood, and then returned to 20 °C for the remainder of the lifespan [13 (link)]. To ensure drug potency, animals were transferred every second day. Experiments were repeated at least twice. The mean, SEM, P value and lifespan value were summarized in Supplementary Table 1 in the Supporting Information.

Longevity assays were conducted at 20°C unless otherwise stated. The synchronized late L4 larvae or young adults were transferred to plates seeded with inactive OP50 (65°C for 30 minutes), 20 μM of 5-fluro-2′-deoxyuridine (FUDR, Sigma) to prevent the hatching of eggs, and the indicated compounds [21 (link)]. This day was defined as test day 0. Nematodes were then transferred to fresh corresponding plates with or without orientin every other day. The death of worms was monitored each day throughout the experiments. If the nematode cannot respond to this external mechanical stimulus (lightly touch the head or tail of the nematode with platinum), it is defined as dead. Worms under situations, such as having escaped from the plate, raised genital pores of nematodes, overflow of intestinal contents, or hatching of larvae from the body, are not counted as dead [21 (link)]. The final counted number of nematodes in each group is guaranteed to be at least 60. The life span experiments were conducted independently at least for three times. Statistical analyses were carried out by SPSS and Kaplan-Meier, and the statistical significance (p value) was calculated by the log-rank (Mantel-Cox) test.