AM251(N-(piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide),WIN55,212-2([(3R)-2,3-dihydro-5-methyl-3-(4 morpholinylmethyl)pyrrolo[1,2,3-de]-1,4-benzoxazin-6-yl]-1-naphthalenyl-methanone) and streptozotocin (STZ) (2-deoxy-2-[[(methylnitrosoamino)carbonyl] amino]-D-glucose) were purchased from Cayman Chemical. Rimonabant (biaryl pyrazole N-(piperidinyl)-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide) was synthesized at Ajou University. Antibodies against caspase-3, cyclin D2, and Bcl-2 were purchased from Cell Signaling Technology. An antibody against β-actin was purchased from Abcam. Antibody against Bax was purchased from Santa Cruz. Anti-mouse and anti-rabbit secondary horseradish peroxidase conjugate (HRP) was obtained from Bio-Rad Laboratories.
Antibody against bax
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Antibody against Bax is a laboratory reagent used to detect the Bax protein in biological samples. Bax is a pro-apoptotic member of the Bcl-2 family of proteins and plays a role in the regulation of apoptosis, or programmed cell death. This antibody can be used in various analytical techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and localization of Bax in different cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Streptozotocin (stz), by Cayman Chemical (1 mentions)
Antibody against β actin, by Abcam (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Am251, by Cayman Chemical (1 mentions)
Win55 212 2, by Cayman Chemical (1 mentions)
Hela cell, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Atg12, by Santa Cruz Biotechnology (1 mentions)
Antibody against caspase 3, by Cell Signaling Technology (1 mentions)
Antibody against bcl 2, by Abcam (1 mentions)
2 protocols using antibody against bax
1
Molecular Mechanisms of Cannabinoid Signaling
2
HeLa Cell Autophagy Induction by S1
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human cervical cancer cells (HeLa cells) were obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA) and cultured in Iscove’s modified Dulbecco’s media (IMDM) (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% (v/v) FBS (Thermo Fisher Scientific) at 37°C with 5% CO2. When S1 was used to treat the HeLa cells, the concentration was 10 μM/L in IMDM. The antibody against Bcl-2 (1:500, rabbit) and LC3 (1:500, rabbit) was purchased from Abcam (Cambridge, MA, USA). The antibody against Bax (1:100, mouse) and Atg 12 (1:100, rabbit) was purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). The antibody against caspase-3 (1:500, rabbit) was purchased from Cell Signaling (Beverly, MA, USA). The antibody against Beclin (1:100, mouse) was purchased from BD Biosciences (San Jose, CA, USA).
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