Total RNA was extracted using E.Z.N.A. Plant RNA Kit (Omega). DNA was removed by RQ1 RNase-Free DNase (Promega). rRNA was depleted using riboPOOLs (siTOOLs Biothch) and Streptavidin magpoly beads (Smart-Lifesciences). The resulting total RNA was used to construct the strand-specific sequencing library with NEBNext Ultra II Directional RNA Library Prep Kit (NEB) for Illumina (Nova Seq 6000, PE150) sequencing. The repeatability of sequencing data is shown in Additional file 1 : Fig. S11. A summary of sequencing data is presented in Additional file 2 .
Streptavidin magpoly beads
Manufactured by Smart-Lifesciences
Streptavidin magpoly beads are magnetic beads with streptavidin coated on the surface. Streptavidin is a protein that has a high affinity for biotin, a vitamin commonly used in biological research and applications.
Automatically generated - may contain errors
Lab products found in correlation
Nebnext ultra 2 directional rna library prep kit, by New England Biolabs (1 mentions)
Novaseq 6000, by Illumina (1 mentions)
Rq1 rnase free dnase, by Promega (1 mentions)
Rnasezap, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Rneasy rna purification kit, by Qiagen (1 mentions)
Rnase inhibitor, by Promega (1 mentions)
Low melting point agarose, by Thermo Fisher Scientific (1 mentions)
Tbe buffer, by Thermo Fisher Scientific (1 mentions)
Perfectstart green qpcr supermix, by Transgene (1 mentions)
Rnasecure reagent, by Thermo Fisher Scientific (1 mentions)
Turbo dnase, by Thermo Fisher Scientific (1 mentions)
Mrna capture beads, by Vazyme (1 mentions)
Yeast trna, by Roche (1 mentions)
2 protocols using streptavidin magpoly beads
1
RNA-seq Library Preparation Protocol
2
Comprehensive RNA Isolation and Library Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Yeast-tRNA (Roche 10109525001), RNase inhibitor (Promega N251A), RNaseZap (Invitrogen AM9780), TRIzol Reagent (Invitrogen 15596018), 3 M Sodium Acetate pH5.5 (Invitrogen AM9740), Perfect Start Green qPCR SuperMix (TransGene AQ601-04), RNeasy RNA purification Kit (Qiagen 74104), mRNA Capture Beads (Vazyme N401), Qubit RNA Assay Kit (Qubit Q32852), RNA secure Reagent (Ambion AM7006), Turbo DNase (Invitrogen AM2238), riboPOOLs (rRNA removal probes) (siTOOLs Biotech), Streptavidin magpoly beads (SMART lifesciences SM01710), Low Melting Point Agarose (Invitrogen 16520-050), 10 × TBE Buffer (Invitrogen 15581-044), DNA size Marker (DL1000, Takara 3591Q), NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!