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Elfn2

Manufactured by Merck Group
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ELFN2 is a specialized laboratory equipment designed for specific scientific applications. It functions as a core component in various experimental setups, providing essential capabilities to researchers and scientists. The detailed specifications and intended uses of this product are not available in this factual, unbiased description.

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Lab products found in correlation

Psd95, by Cell Signaling Technology (2 mentions) Glua1, by Abcam (2 mentions) C myc, by GenScript (2 mentions) Mglur8, by GeneTex (2 mentions) Mglur4, by Merck Group (2 mentions) Mglur2, by Santa Cruz Biotechnology (2 mentions) Mglur5, by Merck Group (2 mentions) Gapdh, by Merck Group (2 mentions) Aurka, by Cell Signaling Technology (1 mentions) Atg12, by Cell Signaling Technology (1 mentions) Beclin 1, by Cell Signaling Technology (1 mentions) Phospho aurka, by Cell Signaling Technology (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Gapdh, by Proteintech (1 mentions) Rapamycin, by MedChemExpress (1 mentions)

3 protocols using elfn2

1

Antibody Dilutions for Western Blotting

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The following is a list of antibodies used for Western blotting and their dilutions used in 3% milk: GAPDH, Millipore Cat#AB2302 (1:30000); GluA1, Abcam Cat#ab76321 (1:1000); NR1, Zymed Cat#32–500 (1:1000); NR2B, Millipore Cat#AB1557P (1:1000); Rabbit anti-Gβ1, a gift from Dr. Willardson PMID:15485848 (1:6000); Synaptophysin, Assay Biotech Cat#C0333 (1:1000); PSD-95, Cell Signaling Cat# 3450 (1:1000); GABAARα1, Neuromab Cat#75–136 (1:1000); c-Myc, GenScript Cat#A00172 (1:1000); ELFN2, Sigma Cat#HPA000781 (1:1000); mGluR7, Upstate Cat#07–239 (1:2000); mGluR8 GeneTex Cat#GTX82530 (1:500); mGluR4, EMD Millipore Cat#AB15097 (1:2000); mGluR2, Santa Cruz Cat#sc-271655 (1:500); mGluR5, Millipore Cat#06451. Secondary antibodies were purchased from Jackson ImmunoResearch and used at 1:7500 in 3% milk. Other specific or unique materials used are listed under appropriate methods sections.
Dunn H.A., Zucca S., Dao M., Orlandi C, & Martemyanov K.A. (2019). ELFN2 is a postsynaptic cell adhesion molecule with essential roles in controlling group III mGluRs in the brain and neuropsychiatric behavior. Molecular psychiatry, 24(12), 1902-1919.
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2

Autophagy-related Protein Analysis

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The reagents, chemicals, and antibodies used in this study are as follows: LC3 (Cell Signaling Technology, 12741), Beclin-1 (Cell Signaling Technology, 3495), Atg5 (Cell Signaling Technology, 12994), Atg12 (Cell Signaling Technology, 4180), Atg7 (Cell Signaling Technology, 8558), Atg3 (Cell Signaling Technology, 3415), GFP (Proteintech, 50430-2-AP), ELFN2 (Sigma, HPA00781), phospho-AurkA (Cell Signaling Technology), AurkA (Cell Signaling Technology, 14475), glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Proteintech, 60004-1-Ig), FLAG (Sigma-Aldrich, F3165), and rapamycin (MedChemExpress, HY-10219).
Liu C., Fu H., Liu X., Lei Q., Zhang Y., She X., Liu Q., Liu Q., Sun Y., Li G, & Wu M. (2018). LINC00470 Coordinates the Epigenetic Regulation of ELFN2 to Distract GBM Cell Autophagy. Molecular Therapy, 26(9), 2267-2281.
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3

Antibody Dilutions for Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following is a list of antibodies used for Western blotting and their dilutions used in 3% milk: GAPDH, Millipore Cat#AB2302 (1:30000); GluA1, Abcam Cat#ab76321 (1:1000); NR1, Zymed Cat#32–500 (1:1000); NR2B, Millipore Cat#AB1557P (1:1000); Rabbit anti-Gβ1, a gift from Dr. Willardson PMID:15485848 (1:6000); Synaptophysin, Assay Biotech Cat#C0333 (1:1000); PSD-95, Cell Signaling Cat# 3450 (1:1000); GABAARα1, Neuromab Cat#75–136 (1:1000); c-Myc, GenScript Cat#A00172 (1:1000); ELFN2, Sigma Cat#HPA000781 (1:1000); mGluR7, Upstate Cat#07–239 (1:2000); mGluR8 GeneTex Cat#GTX82530 (1:500); mGluR4, EMD Millipore Cat#AB15097 (1:2000); mGluR2, Santa Cruz Cat#sc-271655 (1:500); mGluR5, Millipore Cat#06451. Secondary antibodies were purchased from Jackson ImmunoResearch and used at 1:7500 in 3% milk. Other specific or unique materials used are listed under appropriate methods sections.
Dunn H.A., Zucca S., Dao M., Orlandi C, & Martemyanov K.A. (2019). ELFN2 is a postsynaptic cell adhesion molecule with essential roles in controlling group III mGluRs in the brain and neuropsychiatric behavior. Molecular psychiatry, 24(12), 1902-1919.
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