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Okadaic acid oa

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Okadaic acid (OA) is a naturally occurring marine biotoxin produced by certain species of dinoflagellates. It is a potent inhibitor of protein phosphatases types 1 and 2A, which play crucial roles in cellular signaling pathways. OA is commonly used as a research tool in cell biology and biochemistry studies.

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Lab products found in correlation

Phosphatase inhibitor cocktails 1 and 2, by Merck Group (1 mentions) Recombinant human tgf β1, by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions)

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Okadaic acid (134 citations)

2 protocols using okadaic acid oa

1

Measuring Tau and Beta-Tubulin in iPSC-Derived Cortical Networks

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iPSC-derived cortical networks were treated with 50 nM Okadaic acid (OA; Merck Life Science) for 2 hours at 37 °C in 5% CO2. Afterward, untreated and treated neuronal cultures were incubated with either 100 μM TAU1 probe or 100 μM BT1 probe for 30 minutes at 37 °C and thus fixed for 15 minutes at room temperature with cool and fresh-made 4% PFA. Note that we did not observed neurotoxicity when using 100 µM BT1 and TAU1.
Soloperto A., Quaglio D., Baiocco P., Romeo I., Mori M., Ardini M., Presutti C., Sannino I., Ghirga S., Iazzetti A., Ippoliti R., Ruocco G., Botta B., Ghirga F., Di Angelantonio S, & Boffi A. (2022). Rational design and synthesis of a novel BODIPY-based probe for selective imaging of tau tangles in human iPSC-derived cortical neurons. Scientific Reports, 12, 5257.
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2

Quantifying Transforming Growth Factor-Beta Signaling

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Cultured cell lines were obtained from the Japanese Collection of Research Bioresources Cell Bank. Recombinant human TGF-β1 was purchased from PeproTech. Protease inhibitor cocktail, for use with mammalian cell and tissue extracts, and phosphatase inhibitor cocktails 1 and 2 were purchased from Sigma-Aldrich. The Protein kinase inhibitor (PKA), H-89 was obtained from Santa Cruz Biotechnology Inc. and okadaic acid (OA) was procured from Merck (Calbiochem, KGaA). All other purchased reagents were of analytical grade.
Hirano T., Saito D., Yamada H., Ishisaki A, & Kamo M. (2020). TGF-β1 induces N-cadherin expression by upregulating Sox9 expression and promoting its nuclear translocation in human oral squamous cell carcinoma cells. Oncology Letters, 20(1), 474-482.
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