Cellbind surface cell culture plates

Manufactured by Corning

Sourced in United States

CellBIND® Surface cell culture plates are a product line offered by Corning, a leading manufacturer of laboratory equipment. These plates are designed for cell culture applications, providing a specialized surface that supports the attachment and growth of various cell types.

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Lab products found in correlation

William s e medium, by Thermo Fisher Scientific (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Stempro msc sfm xenofree, by Thermo Fisher Scientific (1 mentions) Raw 264, by Korean Cell Line Bank (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CellBIND Surface plates CellBIND culture plates CellBIND surface CellBIND plates Cell culture plates CellBIND

2 protocols using cellbind surface cell culture plates

Silencing USF1 in Liver Cell Lines

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The most effective USF1 silencing shRNA was screened in immortalized human hepatocytes (IHH). IHH were grown in William’s E medium (GIBCO-Life Technologies, Carlsbad, CA) containing 10% fetal bovine serum (FBS), 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, 100 nM (20 mU/mL) insulin, and 50 nM dexamethasone (Sigma-Aldrich, St. Louis, MO) on CellBIND® Surface cell culture plates (Corning, Corning, NY). The human monocytic cell line, THP-1 and human hepatocellular carcinoma cell line, HuH-7 were cultured in RPMI (THP-1) or DMEM (HuH-7) medium supplemented with 10% fetal bovine serum and penicillin/streptomycin and to THP-1 media 25 mM HEPES was added. For transduction, 20,000 THP-1 cells seeded on 24-well plates or ~ 80% confluent HuH-7 cells in 12-well plates were treated for 24 h with SIGMA MISSION lentiviral preparations containing either the control shRNA expression vector (MISSION® pLKO.1-puro Non-Target shRNA) or the USF1 silencing shRNA expression vector (233475) at a MOI of ~ 1. Cells were selected with 6 μg/mL of puromycin for 14 days and were then used for cholesterol efflux assays.
Cell lines were found to be free of Mycoplasma contamination.

Ruuth M., Soronen J., Kaiharju E., Merikanto K., Perttilä J., Metso J., Lee-Rueckert M., Taskinen M.R., Kovanen P.T., Öörni K., Olkkonen V.M., Jauhiainen M.S, & Laurila P.P. (2018). USF1 deficiency alleviates inflammation, enhances cholesterol efflux and prevents cholesterol accumulation in macrophages. Lipids in Health and Disease, 17, 285.

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Immunomodulation by rhBMP-2-primed Stem Cells

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Human ES-MSCs were provided by Daewoong Pharmaceutical Co., Ltd. ES-MSCs were cultured in a StemPro^® MSC SFM XenoFree (Gibco, Waltham, MA, USA) supplemented with 1% L-glutamine (Gibco) and 1% penicillin/streptomycin on the Corning^® CellBIND^® surface cell culture plates (Corning Inc., Corning, NY, USA) at 37°C. For priming with rhBMP-2, cells were treated with rhBMP-2 (100 ng/ml) for 24 h. All experiments were performed using ES-MSCs at passage number 12. RAW264.7, murine macrophage cell line, was purchased from Korean Cell Line Bank (KCLB, Seoul, Korea). RAW264.7 cells were grown in RPMI-1640 medium at 37°C. To determine immunomodulation effect by paracrine factors from rhBMP-2-primed ES-MSCs in RAW264.7, cells were seeded in 60 mm culture dish at confluency 70%–80%. RAW264.7 cells were incubated with conditioned medium (CM) from ES-MSCs/rhBMP-2-primed ES-MSCs mixed with RPMI1640 (2:1 ratio). After 2 h, LPS was added for 6 h. Cells were lysed with RIPA buffer for western blotting analysis, and CM was used for ELISA.

Lee J., Jang J., Cha S.R., Lee S.B., Hong S.H., Bae H.S., Lee Y.J, & Yang S.R. (2023). Recombinant Human Bone Morphogenetic Protein-2 Priming of Mesenchymal Stem Cells Ameliorate Acute Lung Injury by Inducing Regulatory T Cells. Immune Network, 23(6), e48.

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