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Cd140b

Manufactured by Miltenyi Biotec
Sourced in Germany

CD140b is a cell surface marker that is expressed on certain cell types. It can be used to identify and isolate these cells for research purposes.

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4 protocols using cd140b

1

Mesenchymal Stem Cell Migration in Acute Lung Injury

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The surface markers of MenSCs including CD140b, CD184, CD185, CD191, CD192, CD194, CD197, CD202b and CD221 (Miltenyi, Teterow, Germany) were detected by flow cytometry.
MenSCs (3 × 104/mL in 100 µL) were seeded on 24-well inserts and BEAS-2B cells were seeded in the 24-well culture plate wells. After the cells became adherent, the medium was replaced with α-MEM without fetal bovine serum (FBS). At a final concentration of 100 ng/mL, LPS was added to the basal chambers of the migration group. Phosphate-buffered saline (PBS) was served as a negative control, and α-MEM with 30% FBS served as a positive control. After 24 h, the cells were stained with Dil for observation by fluorescence microscope.
C57 mice were divided into a control group and an ALI group and anesthetized. LPS at a dosage of 5 mg/kg and the same volume of PBS were instilled intratracheally into the lungs of the migration group and the control group. After 4 h, 1 × 106 MenSCs labeled with the luciferase gene were injected into the mice tail vein and the mice were observed using a live imaging system for 3 days.
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2

Multiparametric Flow Cytometry Characterization

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FACS analysis and cell sorting were performed using FACS Calibur and FACS Aria instruments (Becton Dickinson, Palo Alto, CA), respectively. FACS data were analyzed using FlowJo software (Tree Star, Ashland, OR). Antibodies against the following proteins were used: PE-conjugated CD34 (MACS; Miltenyi Biotech, 30-081-002), CD44 (MACS; Miltenyi Biotech, 130-095-180), CD45 (MACS; Miltenyi Biotech, 130-080-201), CD73 (MACS; Miltenyi Biotech, 130-095-182), CD105 (MACS; Miltenyi Biotech, 130-094-941), CD140b (MACS; Miltenyi Biotech, 130-105-279), and W5C5 (MACS; Miltenyi Biotech, 130-111-641). The FACS gates were established by staining with an isotype antibody or secondary antibody.
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3

Comprehensive Phenotypic Characterization of Cells

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FACS analysis and cell sorting were performed using FACSCalibur and FACS Aria machines (Becton Dickinson, Palo Alto, CA), respectively. FACS data were analyzed using FlowJo software (Tree Star, Ashland, OR). Antibodies to the following proteins were used: PE-conjugated CD34 (MACS; Miltenyi Biotec, 30-081-002), CD44 (MACS; Miltenyi Biotec, 130-095-180), CD45 (MACS; Miltenyi Biotec, 130-080-201), CD73 (MACS; Miltenyi Biotec, 130-095-182), CD105 (MACS; Miltenyi Biotec, 130-094-941), CD140b (MACS; Miltenyi Biotec, 130-105-279), and W5C5 (MACS; Miltenyi Biotec, 130-111-641). The FACS gates were established by staining with an isotype antibody or secondary antibody.
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4

Multiparametric Immunophenotyping of Stem Cells

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FACS analysis and cell sorting were performed using FACS Calibur and FACS Aria machines (Becton Dickinson, Palo Alto, CA), respectively. FACS data were analyzed using FlowJo software (Tree Star, Ashland, OR). Antibodies against the following proteins were used: APC-conjugated CD44 (BD Bioscience, Cat. 559942, dilution 1/40), PE-conjugated CD133 (MACS; Miltenyi Biotech, 130-080-081, dilution 1/40), CD34 (MACS; Miltenyi Biotech, 30-081-002), CD44 (MACS; Miltenyi Biotech, 130-095-180), CD45 (MACS; Miltenyi Biotech, 130-080-201), CD73 (MACS; Miltenyi Biotech, 130-095-182), CD105 (MACS; Miltenyi Biotech, 130-094-941), and CD140b (MACS; Miltenyi Biotech, 130-105-279). The FACS gates were established by staining with an isotype antibody or secondary antibody.
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