The surface markers of MenSCs including CD140b, CD184, CD185, CD191, CD192, CD194, CD197, CD202b and CD221 (Miltenyi, Teterow, Germany) were detected by flow cytometry.
MenSCs (3 × 104/mL in 100 µL) were seeded on 24-well inserts and BEAS-2B cells were seeded in the 24-well culture plate wells. After the cells became adherent, the medium was replaced with α-MEM without fetal bovine serum (FBS). At a final concentration of 100 ng/mL, LPS was added to the basal chambers of the migration group. Phosphate-buffered saline (PBS) was served as a negative control, and α-MEM with 30% FBS served as a positive control. After 24 h, the cells were stained with Dil for observation by fluorescence microscope.
C57 mice were divided into a control group and an ALI group and anesthetized. LPS at a dosage of 5 mg/kg and the same volume of PBS were instilled intratracheally into the lungs of the migration group and the control group. After 4 h, 1 × 106 MenSCs labeled with the luciferase gene were injected into the mice tail vein and the mice were observed using a live imaging system for 3 days.
MenSCs (3 × 104/mL in 100 µL) were seeded on 24-well inserts and BEAS-2B cells were seeded in the 24-well culture plate wells. After the cells became adherent, the medium was replaced with α-MEM without fetal bovine serum (FBS). At a final concentration of 100 ng/mL, LPS was added to the basal chambers of the migration group. Phosphate-buffered saline (PBS) was served as a negative control, and α-MEM with 30% FBS served as a positive control. After 24 h, the cells were stained with Dil for observation by fluorescence microscope.
C57 mice were divided into a control group and an ALI group and anesthetized. LPS at a dosage of 5 mg/kg and the same volume of PBS were instilled intratracheally into the lungs of the migration group and the control group. After 4 h, 1 × 106 MenSCs labeled with the luciferase gene were injected into the mice tail vein and the mice were observed using a live imaging system for 3 days.