R523062 and R856932 were purchased from Sigma-Aldrich with Cat # of R523062 and R856932, respectively. Pleconaril was purchased from Sigma-Aldrich (Cat # SML0307). Telaprevir was purchased from Achemblock (Cat # F-4593). Rupintrivir was purchased from Sigma-Aldrich (Cat # PZ0315). Human rhabdomyosarcoma (RD; ATCC CCL-136) was maintained at 37 °C in a 5% CO2 atmosphere and cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin−streptomycin antibiotics. The following reagents were obtained through BEI Resources, NIAID, NIH: Human Enterovirus D68, US/MO/14–18949, NR-49130; Enterovirus D68, US/MO/14– 18947, NR-49129; Enterovirus D68, US/IL/14–18952, NR49131; Enterovirus D68, US/KY/14–18953, NR-49132; Enterovirus D68, US/IL/14–18956, NR-49133; Human Enterovirus A71 (EV-71), Tainan/4643/1998, NR-471; Human Enterovirus A71 (EV-71), MP4, NR-472. Enteroviruses A71 (EV-71) US/CT/2016–19519 and US/AK/2016–19516 strains were obtained from Dr. William Nix at the Centers for Disease Control and Prevention under a material transfer agreement. All viruses were amplified in RD cells prior to infection assays.
Ma C., Hu Y., Zhang J, & Wang J. (2020). Pharmacological characterization of the mechanism of action of R523062, a promising antiviral for enterovirus D68. ACS infectious diseases, 6(8), 2260-2270.
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