Ff01 676 29 25

Manufactured by IDEX Corporation

Sourced in United States

The FF01-676/29-25 is a precision optical filter designed for use in laboratory equipment. It features a narrow bandpass that selectively transmits a specific wavelength of light while blocking all others. The filter's core function is to isolate and purify a desired wavelength for various scientific and analytical applications.

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Lab products found in correlation

Ixon du897 bv, by Oxford Instruments (2 mentions) Observer a1, by Zeiss (1 mentions) Di01 r405 488 561 635 25x36, by IDEX Corporation (1 mentions) Plan apochromat oil, by Zeiss (1 mentions) Axio observer a1 microscope, by Zeiss (1 mentions) Di01 r405 488 561 635 25 36, by IDEX Corporation (1 mentions) Ff01 520 35 25, by IDEX Corporation (1 mentions) Spectralis hra oct, by Heidelberg Engineering (1 mentions) Ichrome mle, by Toptica (1 mentions)

3 protocols using ff01 676 29 25

Super-Resolution Microscopy of BS-C-1 Cells

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Images of BS-C-1 cells were acquired using a standard inverted epifluorescence microscope (Observer A1, Zeiss) fitted with a 63× (1.4 NA) Plan-Apochromat oil-immersion objective (Carl Zeiss). The sample was illuminated using 635 nm and 450 nm diode lasers (OptoEngine) for the excitation of Alexa 647 and for photoswitching respectively. The illumination light was directed towards the microscope using custom laser excitation optics, reflected into the sample using a dichroic filter (Di01-R405/488/561/635-25x36, Semrock), and focused onto the back focal plane of the objective lens. Signal from the sample was filtered using a bandpass filter (FF01-676/29-25, Semrock) and acquired by an electron multiplying charge coupled device (EMCCD) camera (iXon DU897-BV, Andor) set to conventional readout mode. Axial drift during the course of the acquisition was corrected in real time using a custom focus stabilization system comprising an 850 nm diode laser (PI, USA), a quadrant position detector (Thorlabs), and an XYZ piezo positioner (PI, USA). All devices including lasers, shutters, and cameras were controlled and synchronized using custom-written software in the C programming language.

Cohen E.A., Abraham A.V., Ramakrishnan S, & Ober R.J. (2019). Resolution limit of image analysis algorithms. Nature Communications, 10, 793.

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Multichannel Fluorescence Microscopy Setup

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Custom laser optics, configured with 635-nm and 405-nm diode lasers (OptoEngine) for the excitation and photoactivation, respectively, of Alexa Fluor 647, were used with a Zeiss Axio Observer.A1 microscope. The lasers were reflected onto the sample using a dichroic filter (Di01-R405/488/561/635-25× 36; Semrock) and focused on the back focal plane of a 63×, 1.46 NA Zeiss objective lens. The fluorescence emitted by Alexa Fluor 647 was collected by the objective lens and filtered with a single bandpass filter (FF01-676/29-25; Semrock). Images were acquired using an electron-multiplying charge-coupled device camera (iXon DU897-BV; Andor) in conventional readout mode. The pixel size of the camera was 16 μm × 16 μm. Custom software written in the C programming language was used to control and synchronize the various components, including the lasers, shutters, and the camera.

Vahid M.R., Chao J., Ward E.S, & Ober R.J. (2017). A state space based approach to localizing single molecules from multi-emitter images. Proceedings of SPIE--the International Society for Optical Engineering, 10070, 100700J.

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Adaptive Optics Imaging of Retinal Cells

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Mice were imaged with a custom adaptive optics scanning light ophthalmoscope (AOSLO), using near-infrared light (796∆17 nm, 200-500 µW, super luminescent diode: S790-G-I-15, Superlum, Ireland) [2, 17] . Phase-contrast imaging referred to in the context of this paper, was achieved by purposefully displacing the detector axially to a plane conjugate to the highly reflective RPE/choroid complex, to enable detection of forward and multiply scattered light from translucent cells, as detailed in our recent publication [5] . In a subset of experiments for confirmation of immune cell types, fluorescence was simultaneously imaged using 488 nm excitation and 520Δ35 emission for GFP, and 640 nm excitation and 676Δ29 emission for Alexa Fluor 647 (excitation laser diode: iChrome MLE, Toptica Photonics, Farmington, New York, USA; emission filters: FF01-520/35-25 and FF01-676/29-25, Semrock, Rochester, New York, USA). Mice also underwent imaging with HRA+OCT Spectralis (Heidelberg Engineering, Germany).

Joseph A., Chu C.J., Feng G., Dholakia K., & Schallek J. (2020). Label-free imaging of immune cell dynamics in the living retina using adaptive optics.

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