Penicillin 100 u streptomycin 100 g solution

Human embryonic kidney cells (HEK‐293T, ATCC: CRL‐11268) were cultivated in Dulbecco's modified Eagle's medium (DMEM; Thermo Fischer Scientific, cat. no. 10566016) supplemented with 10% fetal bovine serum (Sigma Aldrich, cat. no. F7524, lot no. 022M3395) and penicillin (100 U)‐streptomycin (100 µg) solution (Sigma Aldrich, cat. no. P433) under a humidified atmosphere of 5% CO₂ in air at 37 °C. Passaging of pre‐confluent HEK‐293 cultures was performed by trypsinization with 0.05% trypsin‐EDTA (Life Technologies, Carlsbad, CA, USA; cat. no. 25300‐054) for 5 min at 37 °C. Cells were transferred to 10 mL cell culture medium, and centrifuged for 1 min at 200 × g. The supernatant was discarded and the cells were resuspended in fresh medium. Cell number and viability were quantified using an electric field multichannel cell‐counting device (Casy Cell Counter and Analyzer Model TT, Roche Diagnostics GmbH, Rotkreuz, Switzerland).

Human embryonic kidney cells (HEK-293T, ATCC: CRL-3216) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific, cat. no. 10566016) supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich, cat. no. F7524, lot no. 022M3395) and penicillin (100 U)-streptomycin (100 µg) solution (Sigma-Aldrich, cat. no. P433) under a humidified atmosphere of 5% CO₂ in air at 37 °C. Passaging of pre-confluent HEK-293T cultures was performed by trypsinization with 0.05% trypsin-EDTA (Life Technologies, Carlsbad, CA, USA; cat. no. 25300-054) for 5 min at 37 °C. Cells were transferred to 10 ml cell culture medium, and centrifuged for 1 min at 200 × g. The supernatant was discarded and the cells were resuspended in a fresh medium. Cell number and viability were quantified using an electric field multichannel cell-counting device (Casy® Cell Counter and Analyzer Model TT, Roche Diagnostics GmbH, Rotkreuz, Switzerland).